高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

白藜芦醇白蛋白纳米粒的制备及其抗卵巢癌细胞增殖作用的研究

郭莉媛, 姚晶萍, 隋丽华   

  1. 哈尔滨医科大学附属肿瘤医院妇科, 哈尔滨 150040
  • 收稿日期:2008-10-31 修回日期:1900-01-01 出版日期:2009-03-10 发布日期:2009-03-10
  • 通讯作者: 隋丽华

Preparation and Effects of Resveratrol Bovine Serum Albumin Nanoparticles on Proliferation of Human Ovarian Carcinoma Cell SKOV3

GUO Li-Yuan, YAO Jing-Ping, SUI Li-Hua*   

  1. Gynecology Department of Harbin Tumor Hospital, Harbin Medical University, Harbin 150040, China
  • Received:2008-10-31 Revised:1900-01-01 Online:2009-03-10 Published:2009-03-10
  • Contact: SUI Li-Hua

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被引次数

19

摘要： 以牛血清白蛋白(BSA)为载体, 用去溶剂化-化学交联法制备白藜芦醇白蛋白纳米粒(RES-BSANP). 以原子力学显微镜(AFM)观察其形态, 用高效液相色谱法(HPLC)对制备的纳米微粒进行分析. 采用四甲基偶氮唑盐微量酶反应比色法(MTT)及流式细胞技术(FCM)比较RES-BSANP和RES对卵巢癌SKOV3细胞的抗增殖活性及对细胞周期和凋亡的影响. 结果表明, 获得的RES-BSANP纳米粒的平均粒径为400～500 nm, 表面光滑, 12 mg纳米粒中RES载药量为4.077 mg, 包封率33.97％, 24 h内的稳定性好, 水溶性较RES显著提高. 二者的抗肿瘤增殖作用呈剂量依赖性, 中高浓度组纳米粒组的抗增殖活性及凋亡细胞比率显著提高. 两种药物均使细胞周期阻滞于G0/G1+S期, 纳米组使进入S期细胞比率明显增加, 表明白藜芦醇白蛋白纳米粒在抗卵巢癌细胞增殖方面有广阔的应用前景.

关键词:

白藜芦醇(RES), 牛血清白蛋白(BSA), 白藜芦醇白蛋白纳米粒(RES-BSANP)

Abstract: Resveratrol bovine serum albumin nanoparticles(RES-BSANP) were synthesized by desolvation. HPLC and Atomic force microscopy(AFM) were employed to study the shape, mean size and particle distribution of RES-BSANP. MTT was used to detect the effects of RES and RES-BSANP on the growth rate of cell SKOV3 at various concentration of them, and flow cytometry(FCM) was applied to assaying the cell cycle and cell apoptosis of cells SKOV3. The results show that RES-BSANP are slick and uniformity, the average diameter of RES-BSANP is about 400—500 nm, drug loading is about 4.077 mg/12 mg, entrapment efficiency is 33.97％. MTT test shows the growth of SKOV3 can be significantly inhibited by RES and RES-BSANP in dose-dependence manner, while RES-BSANP is superior to RES at concentrations of 60, 100, 200 μmol/L(P<0.05). FCM test shows RES-BSANP make cells SKOV3 be in S phase and the apoptosis of cells SKOV3 more significant compared with RES. Therefore, RES-BSANP appears to be a very promising drug for anti-proliferation on human ovarian carcinoma.

Key words: Resveratrol(RES), Bovine serum albumin(BSA), Resveratrol bovine serum albumin nanoparticles(RES-BSANP)

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