高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (S1): 160.

• Chemistry in Life Sciences • 上一篇    下一篇

Single Molecule Force Spectroscopy on Proteins by AFM——Nanomechanics Meets Molecular Biology

LI Hong-Rin, OBERHAUSER A., MARSZALEK P., CARRION M., FERNANDEZ J.   

  1. Department of Physiology and Biophysics, Mayo Foundation, Rochester, MN 55905, USA
  • 出版日期:2000-12-31 发布日期:2000-12-31

Single Molecule Force Spectroscopy on Proteins by AFM——Nanomechanics Meets Molecular Biology

LI Hong-Rin, OBERHAUSER A., MARSZALEK P., CARRION M., FERNANDEZ J.   

  1. Department of Physiology and Biophysics, Mayo Foundation, Rochester, MN 55905, USA
  • Online:2000-12-31 Published:2000-12-31

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摘要：

Tandem modular proteins underlie the elasticity of natural adhesives, cell adhesion proteins, and muscle proteins. The fundamental unit of elastic proteins is their individually folded modules. Here, we combine single molecule force spectroscopy and molecular biology to investigate the nanomechanical properties of these modular proteins. Our experiments reveal the mechanical design of modular proteins, and opens the way for the engineering of elastic proteins with defined and tunable mechanical properties, which can be used in tissue and fiber engineering.

Abstract:

Tandem modular proteins underlie the elasticity of natural adhesives, cell adhesion proteins, and muscle proteins. The fundamental unit of elastic proteins is their individually folded modules. Here, we combine single molecule force spectroscopy and molecular biology to investigate the nanomechanical properties of these modular proteins. Our experiments reveal the mechanical design of modular proteins, and opens the way for the engineering of elastic proteins with defined and tunable mechanical properties, which can be used in tissue and fiber engineering.

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