高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (S1): 11.

• Analytical Sciences • 上一篇    下一篇

Ion Channels as Sensing Elements-Investigations by Patch-Clamp Technique

LI Jie, RAUTENBERG Claudia, FRIEDRICH Steffen, SALZER Reiner   

  1. Dresden University of Technology, Institute of Analytical Chemistry, D-01062 Dresden, Germany
  • 出版日期:2000-12-31 发布日期:2000-12-31
  • 通讯作者: SALZER Reiner

Ion Channels as Sensing Elements-Investigations by Patch-Clamp Technique

LI Jie, RAUTENBERG Claudia, FRIEDRICH Steffen, SALZER Reiner   

  1. Dresden University of Technology, Institute of Analytical Chemistry, D-01062 Dresden, Germany
  • Online:2000-12-31 Published:2000-12-31
  • Contact: SALZER Reiner

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摘要：

Ion channels are transmembrane proteins. Their high recognition capability together with an intrinsic amplification effects makes them outstanding candidates as constituents of biosensors. The patch-clamp technique is an extremely powerful and versatile method for studying the functioning of ion channels reconstituted into biological or synthetic membranes. Two well characterized types of natural ion channels were studied, (i) the simple transmembrane protein gramicidin A and (ii) the rather complex ligand-gated nicotinic acetylcholine receptor.

Abstract:

Ion channels are transmembrane proteins. Their high recognition capability together with an intrinsic amplification effects makes them outstanding candidates as constituents of biosensors. The patch-clamp technique is an extremely powerful and versatile method for studying the functioning of ion channels reconstituted into biological or synthetic membranes. Two well characterized types of natural ion channels were studied, (i) the simple transmembrane protein gramicidin A and (ii) the rather complex ligand-gated nicotinic acetylcholine receptor.

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