高等学校化学学报 ›› 2014, Vol. 35 ›› Issue (8): 1658-1664.doi: 10.7503/cjcu20140336

• 有机化学 • 上一篇    下一篇

Ⅰ型胶原膜的类液晶结构对人脐带间充质干细胞黏附、增殖和分化性能的影响

曾庆慧1, 李娜1, 田冶2,3, 吴狄1, 赵要武1, 李立华1, 周长忍1()   

  1. 1. 暨南大学材料科学与工程系, 广州 510632
    2. 广东省医疗器械质量监督检验所, 3. 广州市生物医用血液净化材料研究与开发重点实验室, 广州 510663
  • 收稿日期:2014-04-10 出版日期:2014-08-10 发布日期:2014-06-10
  • 作者简介:联系人简介: 周长忍, 男, 博士, 教授, 主要从事生物材料领域的研究. E-mail: tcrz9@jnu.edu.cn
  • 基金资助:
    国家自然科学基金(批准号: 31270021, 81171459)、 广东省自然科学基金(批准号: S2013010013955)、 珠江新星专项项目(批准号: 2011J2200037)和暨南大学科研创新基金(批准号: 21612320)资助

Effects of Collagen Films with Liquid Crystal-liked Ordered Structure on Adhesion, Proliferation and Differentiation of Human Umbilical Cord Mesenchymal Stem Cells

ZENG Qinghui1, LI Na1, TIAN Ye2,3,*, WU Di1, ZHAO Yaowu1, LI Lihua1, ZHOU Changren1,*()   

  1. 1. Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, China
    2. Institute of Guangdong Medical Equipment Quality Supervision and Inspection, 3. Guangzhou Biomedical Blood Purification Materials Research and Development Key Laboratory, Guangzhou 510663, China
  • Received:2014-04-10 Online:2014-08-10 Published:2014-06-10
  • Contact: TIAN Ye,ZHOU Changren E-mail:tcrz9@jnu.edu.cn
  • Supported by:
    Supported by the National Natural Science Foundation of China(Nos.31270021, 81171459), the Natural Science Foundation of Guangdong Province, China(No;S2013010013955), the Project of Science & Technology Star of Zhujiang City, Guangdong Province, China(No.2011J2200037) and the Scientific Research Foundation of Jinan University Innovation of China(No.21612320)

摘要:

模拟天然组织中Ⅰ型胶原的有序排列, 利用高浓度胶原的液晶态获得表面具有类液晶有序结构的胶原膜, 并通过体外培养考察其对人脐带间充质干细胞(hUCMSCs)生物性能的影响. 结果表明, 当Ⅰ型胶原膜浓度为120 mg/mL时, 膜表面的胶原纤维呈定向有序排列, 这种有序结构不仅有利于hUCMSCs的黏附和增殖, 而且能促

关键词: Type Ⅰ, collagen, Liquid crystal-liked ordered structure, Human umbilical cord mesenchymal stem cell(hUCMSC), Osteogenic differentiation

Abstract:

To mimic the ordered arrangement of type Ⅰ collagen in natural tissue, a collagen films with liquid crystal-liked ordered surface were fabricated utilizing the liquid crystal state of concentrated collagen. And the effects of the topography on the behavior of human umbilical cord mesenchymal stem cells(hUCMSCs) were studied in vitro. The results showed that the collagen fibrils arranged directly on the surface of the collagen films when the concentration was over 120 mg/mL. This ordered structure improved significantly the adhesion and proliferation of hUCMSCs. In addition, it also increased the expression of osteogenic gene such as ALP, Collagen Ⅰ , RUNX2, Osterix, OCN and OPN, which meant that hUCMSCs had a stronger differentiation potency on the ordered surface than on the unordered surface.

Key words: Type Ⅰ, collagen, Liquid crystal-liked ordered structure, Human umbilical cord mesenchymal stem cell(hUCMSC), Osteogenic differentiation