高等学校化学学报

高等学校化学学报

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钌配合物[Ru(bpy)2(PNT)]2+的合成、表征及与DNA相互作用研究

孙斌, 梁思敏, 王宇传, 巢晖, 计亮年   

  1. 中山大学化学与化学工程学院, 生物无机与合成化学教育部重点实验室, 广州 510275
  • 收稿日期:2008-10-06 修回日期:1900-01-01 出版日期:2008-12-10 发布日期:2008-12-10
  • 通讯作者: 巢晖, 计亮年

Synthesis, Characterization and DNA-Binding Studies of Ruthenium Complex [Ru(bpy)2(PNT)]2+

SUN Bin, LIANG Si-Min, WANG Yu-Chuan, CHAO Hui*, JI Liang-Nian*   

  1. School of Chemistry and Chemical Engineering, Key Laboratory of Bioinorganic and Synthetic Chemistry of Ministry of Education, Sun Yat-Sen University, Guangzhou 510275, China
  • Received:2008-10-06 Revised:1900-01-01 Online:2008-12-10 Published:2008-12-10
  • Contact: CHAO Hui, JI Liang-Nian

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被引次数

19

摘要： 以cis-Ru(bpy)2Cl2·2H2O与PNT为原料合成钌(Ⅱ)多吡啶配合物[Ru(bpy)2(PNT)]2+(bpy=2,2’-联吡啶, PNT=2-[4’-(5-四唑基)苯基]咪唑-[4,5-f][1,10]邻菲咯啉), 通过元素分析、质谱和核磁共振波谱对该化合物进行了结构表征. 利用紫外-可见吸收光谱、荧光光谱、热变性和黏度实验研究了配合物与CT-DNA的相互作用, 实验结果表明, 该配合物以部分插入模式与DNA结合.

关键词: 钌(Ⅱ)配合物, 小牛胸腺DNA, 部分插入

Abstract: A novel ruthenium complex [Ru(bpy)2(PNT)]2+ (bpy=2,2’-bipyridine, PNT=2-[4’-(1H-tetrazol-5-yl)phenyl]-1H-imidazo[4,5-f][1,10]phenanthroline) was synthesized and characterized by elemental analysis, electrospray mass spectra and 1H NMR. The binding of the complexes with calf thymus DNA was investigated by absorption spectroscopy, luminescence titrations, viscosity measurements and DNA thermal denaturation experiments. The experimental results suggest that [Ru(bpy)2(PNT)]2+ binds with DNA by partial intercalation.

Key words: Ruthenium(Ⅱ) complex, CT-DNA, Partial intercalation

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