高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

大鼠去势后肾上腺与前列腺中Cyp17a1基因的表达

王琬1,2, 何永鑫2, 陈君2, 李雪2, 罗国安1,2, 王义明2   

    1. 华东理工大学药学院, 上海 200237;
    2. 清华大学化学系, 北京 100084
  • 收稿日期:2007-11-09 修回日期:1900-01-01 出版日期:2008-07-10 发布日期:2008-07-10
  • 通讯作者: 罗国安

Cyp17a1 Gene Expression in Rat’s Adrenal and Prostate After Orchiectomy

WANG Wan1,2, HE Yong-Xin2, CHEN Jun2, LI Xue2, LUO Guo-An1,2*, WANG Yi-Ming2   

    1. School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China;
    2. Department of Chemistry, Tsinghua University, Beijing 100084, China
  • Received:2007-11-09 Revised:1900-01-01 Online:2008-07-10 Published:2008-07-10
  • Contact: LUO Guo-An

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被引次数

11

摘要： 通过定量检测大鼠去势前后肾上腺和前列腺中Cyp17a1基因的表达, 试图从分子水平解释去势手术后大鼠机体内雄性激素水平的变化.

关键词: Cyp17a1, 肾上腺, 前列腺, 实时荧光定量PCR

Abstract: In this paper the change law of Cyp17a1 gene expression in the process was studied, in which the rats’ adrenal secretes androgen after orchiectomy. The Cyp17a1 gene expression in adrenal and prostate tissues was detected via real-time fluorescent quantitative PCR on different time points, which were before orchiectomy and 1 week, 2 weeks, 3 weeks, 4 weeks after orchiectomy, respectively. The results indicate that Cyp17a1 gene expression in adrenal would increase after orchiectomy, and reach the maximum when 3 weeks after. That Cyp17a1 gene exists in rat’s prostate tissue was discovered for the first time. The conclusion is that adrenal have the function of compensation, increasing the secretion of androgen after orchiectomy. It is presumed that prostatecan synthesizes DHEA as well.

Key words: Cyp17a1, Adrenal, Prostate, Real-time fluorescent quantitative PCR

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