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Chem. J. Chinese Universities
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Scheme 1 Synthesis of the probe HBT-CN
Fig.1 Photographs of color change(A) and fluorescence response(B) of HBT-CN(5.0×10
-6
mol/L) after adding HS
O
3
-
(2.5×10
-4
mol/L), N
2
H
4
·H
2
O(1×10
-3
mol/L) and other analytes(2.5×10
-3
mol/L)
V
(DMSO):
V
(PBS)=1:4.
Table 1 Amino acid sequences of synthetic polypeptides
Table 2 Real-time PCR primer
Fig.1 SEM images of PLGA/COL scaffolds(A—C), interpore structure of the PLGA/COL scaffolds(D—F) and pure PLGA scaffold(G—I)
Fig.2 Water droplets and contact angles on the surface of different scaffolds(A) PLGA; (B) PLGA/COL; (C) BMP2-MP@PLGA/COL; (D) CBD-BMP2-MP@PLGA/COL.
P
<0.05,
n
=4.
Fig.3 Mechanical properties of the PLGA(
a
), PLGA/COL(
b
), BMP2-MP@PLGA/COL(
c
) and CBD-BMP2-MP@PLGA/COL(
d
) tested at room temperature (A) Typical stress-strain curves; (B) compressive strength.
n
=3,
P
<0.05.
Fig.4 Collagen-binding ability of BMP2-MP and CBD-BMP2-MP detected by immunofluorescence (A) Immunofluorescence image; (B) total signal.
E
x
=488 nm,
E
m
=546 nm, error bars represent standard deviation for
n
=3, *
P
<0.05.
Fig.2 Time-dependent fluorescence intensity changes of HBT-CN(5×10
-6
mol/L) in the presence of N
2
H
4
·H
2
O(1×10
-3
mol/L,
λ
ex
=399 nm,
λ
em
=500 nm, slits: 2.5 nm/5 nm)(A) and HS
O
3
-
(1×10
-3
mol/L,
λ
ex
=399 nm,
λ
em
=458 nm, slits: 2.5 nm/5 nm)(B)
Fig.3 Fluorescence intensity of HBT-CN(5×10
-6
mol/L) changes with different pH values
a
. HBT-CN;
b
. HBT-CN+HS
O
3
-
;
c
. HBT-CN+N
2
H
4
·H
2
O.
Fig.4 Fluorescence intensity of HBT-CN(5×10
-6
mol/L) changes upon gradual addition of N
2
H
4
·H
2
O(0—1.2×10
-3
mol/L)(A) and linear relationship between fluorescence intensity(500 nm) of HBT-CN and concentrations of N
2
H
4
·H
2
O(0—1.05×10
-3
mol/L)[
V
(DMSO):
V
(PBS)=1:4;
λ
ex
=399 nm, slit: 5 nm/5 nm](B)
Fig.5 Fluorescence intensity of HBT-CN(5 μmol/L) changes upon gradual addition of HS
O
3
-
(0—3×10
-4
mol/L)(A) and linear relationship between fluorescence intensity of HBT-CN and concentrations of HS
O
3
-
(0—1.2×10
-4
mol/L) at 458 nm[
V
(DMSO):
V
(PBS)=1:4,
λ
ex
=399 nm, slit: 5 nm/5 nm](B)
Fig.6 Fluorescence spectra of HBT-CN(5.0×10
-6
mol/L) in the presence of HS
O
3
-
, N
2
H
4
·H
2
O and other analytes
Fig.7 Fluorescence response of HBT-CN(5×10
-6
mol/L) in the presence of HS
O
3
-
+anions(A) and N
2
H
4
·H
2
O+others(B)Black bars represent the fluorescent intensity(458 nm) of HBT-CN(5×10
-6
mol/L) in the presence of anions after adding HS
O
3
-
; red bars represent the fluorescent intensity(458 nm) of HBT-CN(5×10
-6
mol/L) in the presence of anions in the absence of HS
O
3
-
. (A) a. HS
O
3
-
; b. C
O
3
2
-
; c. S
2
O
3
2
-
; d. S
2-
; e. S
O
4
2
-
; f. H
2
P
O
4
-
; g. C
2
O
4
2
-
; h. AcO
-
; i.
N
3
-
; j. SCN
-
; k. F
-
; l. Cl
-
; m. Br
-
; n. I
-
; o. N
O
3
-
; p. N
O
2
-
. (B) a. N
2
H
4
·H
2
O; b.GSH; c. Cys; d. Hcy; e. H
2
NCONH
2
; f. C
6
H
8
N
2
; g. NH(CH
3
)
2
; h. CH
3
CH
2
NH
2
; i. K
+
; j. Na
+
; k. N
H
4
+
; l. Mg
2+
; m. Ba
2+
.
Scheme 2 Proposed sensing mechanism of HBT-CN for N
2
H
4
·H
2
O and HS
O
3
-
Fig.1 Structure of HEtHex(TFS)
Fig.2 Stability of oil samples with different mass fractions of HEtHex(TFS) at 25 ℃(A) Ultrasound dispersion just ended; (B) ultrasound dispersion ended after 48 h.
Fig.3 Corrosive photos of oil samples with different mass fractions of HEtHex(TFS) at 25 ℃
Fig.4 Friction coefficient diagram of six kinds of oil samples at 25 ℃
Fig.5 Wear scar diameters of six kinds of oil samples at 25 ℃
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