Collagen binding domain(CBD) was conjugated to bone morphogenetic protein-2 mimetic peptide(BMP2-MP) to prepare collagen-binding BMP2-MP(CBD-BMP2-MP), then, CBD-BMP2-MP was combined with PLGA/collagen(PLGA/COL) composite scaffold for the surface modification of scaffold. SEM, electronic universal testing machine and contact angle measuring instrument were used to evaluate the surface morphology, mechanical strength, hydrophilicity and other material properties of PLGA/COL scaffolds. The binding abilities of CBD-BMP2-MP and BMP2-MP to scaffold materials were evaluated by immunofluorescence analysis. Subsequently, MC3T3-E1 cells were grown on different scaffolds, and cell proliferation, adhesion and osteogenic differentiation on different scaffold were evaluated by CCK-8, fluorescence staining, ARS and qPCR. The results show that the 3D porous scaffold has regular and controllable pore structure, which can create more favorable cell microenvironment for cell growth. The addition of collagen on the scaffold surface improved the hydrophilicity of material, and had no effect on the mechanical properties of material itself, which greatly improved the biocompatibility of the 3D scaffold. Compared with commercial BMP2-MP, CBD-BMP2-MP had better collagen binding ability, which improves the combining capacity of growth factor and PLGA/COL scaffold. The scaffold surface loaded with CBD-BMP2-MP has a strong ability to promote osteoblastic differentiation. MC3T3-E1 cells showed higher calcium deposition capacity, and the levels of osteogenic related gens such as COI-1, Runx2, ALP and OPN were also significantly increased. The results indicated that CBD-BMP2-MP modified 3D porous PLGA/COL scaffold has good biocompatibility and osteogenic activity, and is a promising bone tissue repair material.