Abstract: Liver ferritin of Sphyma zygaena(SZLF) with small-scale preparation was done by electrophoresis approach. SZLF consists of iron core, protein shell, and tunnel across its shell. The pathways for dissociation and recombinant both iron core and protein subunits in SZLF were investigated with transmission electron microscopy. Through dissociation(pH=1.5) of hydrochloric acid and dialysis of separation membrane for preparing ferritin subunits, these free subunits were constructed into apoSZLF under the condition of acid-basis neutralization. In this recombinant process, INS was encapsulated with apoSZLF for constructing a nanometer insulin core(NIC) within the protein shell. The experimental evidence for constructing a NIC-SZLF was revealed by electronic spectrometry, MALDI-TOF MS, and SDS-PAGE, respectively, indicating this novel pathway for a NIC-ferritin encapsulated with the protein subunits and insulin. This technology described can be used to supply an important science evidence for constructing a drug carrier with controlling and releasing characteristics in nanometer peptide core-ferritin.

Key words: Ferritin, Nanometer encapsulated, Nanometer insulin core, Poly peptide, Analysis of mass spectrometry