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Studies on a Chemiluminescent Immunoassay with the Erythrocyte-labeled Antibody

ZHANG Wen-Yan1,2, WEI Jing-Yan1, GUO Jie3, CHEN Gui-Qiu4, XING Cheng3, SHI Li-Hua5, YUAN Wei2, QIAN Li-Na2, QUE Xiao-Dong2, GAO Li2, LIU Meng-Hong2   

    1. College of Pharmaceutical Science, Jilin University, Changchun 130021, China;
    2. Jilin Province Center of Clinical Laboratory, Changchun 130021, China;
    3. College of Public Health, Jilin University, Changchun 130021, China;
    4. China-Japan Union Hospital, Jilin University, Changchun 130031, China;
    5. Linjiang Hospital, Linjiang 134600, China
  • Received:2005-10-28 Revised:1900-01-01 Online:2006-08-10 Published:2006-08-10
  • Contact: WEI Jing-Yan

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Abstract: A novel chemiluminescence immunoassay suitable for the determination of the hepatitis B virus' surface antigen(HBsAg) was studied. Instead of the enzyme-labeled antibody in enzyme-lined immunosorbent assay(ELISA), the erythrocyte-labeled monoclonal anti-HBsAg antibodies were utilized as the second antibody in this double antibody sandwich immunoassay. After the sandwich immunoreaction occurs, the sensitized erythrocyte, having combined with the immune complex made from antigen and the first antibody, suffers from hemolysis in low osmotic solution and releases hemoglobin(Hb). Hb can catalyze the chemiluminescent reaction of luminal by hydrogen peroxide. The intensity of the chemiluminescent reaction was linearly with the concentration of the hepatitis B virus' surface antigen(HBsAg) in the detected samples. This assay can be used to detect 0.5 ng/mL of HBsAg. The 30 healthy donors and 30 patients with hepatitis B were detected for HBsAg with this method and with routine ELISA as the standard. The coincidence rate of the two assays was 97%, which illustrates that the developed chemiluminescence immunoassay with erythrocyte-labeled antibody is suitable in clinical determination.

Key words: Erythrocyte-labeled antibody, Chemiluminescent immunoassay, Hepatitis B virus’ surface antigen

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