Treatment of Naphthalene by Microbial Electrochemical System and the Analysis of Microbial Communities †

doi:10.7503/cjcu20190088

Abstract

Abstract:

Naphthalene of different concentrations(15, 30, 60 mg/L) were adopted as substrate for single-chamber air cathode microbial fuel cell(MFC) reactors. The electricity production performance(power density, polarization curve and cyclic voltammetry curve), naphthalene degradation rate, chemical oxygen demand(COD) and total organic carbon(TOC) degradation rate, and anode and cathode microbial diversity were investigated. The results show that the cyclic voltammetry curve was apparently affected by different concentrations of naphthalene; with the increase in naphthalene concentration, the maximum power density showed a downward trend, and naphthalene had a great influence on the cathode potential of MFC; the maximum power density of naphthalene could reach (645.841±28.08) mW/m ²; the degradation rate of naphthalene by MFC was as high as 100%. Moreover, the degradation rate of COD and TOC by MFC increased with the increase of naphthalene concentration, but the increase rate decreased gradually. On this basis, high-throughput sequencing of MFC anode microbial membranes revealed that Geobacter was a dominant genus with a relative abundance of 81%, while the cathode was mainly based on Aquamicrobium.

Key words: Microbial fuel cell, Naphthalene, Degradation rate, Power density curve, Polarization curve, High-throughput sequencing

CLC Number:

O646
O625

TrendMD:

HUA Tao, LI Shengnan, LI Fengxiang, WANG Haonan. Treatment of Naphthalene by Microbial Electrochemical System and the Analysis of Microbial Communities ^†[J]. Chem. J. Chinese Universities, 2019, 40(9): 1964.

Figures/Tables 10

Fig.1 Standard curve for UV detection of naphthalene

Fig.2 CV curves of MFCs at different naphthalene concentrations

Fig.3 Power density and polarized curves at different naphthalene concentrations

Fig.4 Electrode potential at different naphthalene concentrations

Concentration of naphthalene/ (mg·L^-1)	COD of influent/ (mg·L^-1)	TOC of influent/ (mg·L^-1)	Removal rate(%)
Concentration of naphthalene/ (mg·L^-1)	COD of influent/ (mg·L^-1)	TOC of influent/ (mg·L^-1)	Naphthalene	COD		TOC
15	25600	1380	100		37.37±5.80		21.01
30	27300	3040	96.53±2.06		43.10±12.43		35.20±12.86
60	27900	6260	100		58.90±11.67		57.51±9.15

Fig.5 CLSM images of blank control group(A, C) and experimental group(B, D)(40×objective) (A, B) Anode; (C, D) cathode. Concentration of naphthalene/(mg·L-1): (A, C) 0; (B, D) 60.

Fig.6 Rarefaction curves of anode(A) and cathode(B) biofilms

Sample	Shannon index	Simpson index
1	4.179	0.837
2	2.642	0.655
3	6.137	0.97
4	4.792	0.903

Fig.7 Relative abundance histogram at class level (A) Blank control group of anode; (B) experimental group of anode; (C) blank control group of cathode; (D) experimental group of cathode.

Fig.8 Relative abundance histogram at genus level (A) Anode; (B) cathode.

References 40

[1]	Villanueva F., Sevilla G., Lara S., Martín P., Salgado S., Albaladejo J., Cabañas B., ,. Microchem. J. 2018, 142, 117— 125
[2]	Wang J., Song X., Li Q., Bai H., Zhu C., Weng B., Yan D., Bai J., ,. Water. Res. 2019, 150, 340— 348
[3]	Xu X., Xiao R., Dionysiou D. D., Spinney R., Fu T., Li Q., Wang Z., Wang D., Wei Z., ,. Chem. Eng. J. 2018, 351, 248— 257
[4]	Fan B., Wang X. N., Huang Y., Li J., Gao X. Y., Li W. W., Liu Z. T., ,Environ. Sci., 2019,40( 5), 2101— 2114
	(范博, 王晓南, 黄云, 李霁, 高祥云, 李雯雯, 刘征涛.环境科学,2019,40(5), 2101— 2114)
[5]	Al-Mamun A., Ahmad W., Baawain M. S., Khadem M., Dhar B. R., ,. J. Clean. Prod. 2018, 183, 458— 480
[6]	Edokpayi J. N., Odiyo J. O., Popoola O. E., Msagati T. A ., Int. J. Environ. Res. Public Health 2016, 13, 387— 398
[7]	Goswami L., Kumar R. V., Manikandan N. A., Pakshirajan K., Pugazhenthi G., ,. J. Water Process. Eng. 2017, 17, 1— 10
[8]	Goswami L., Manikandan N. A., Dolman B., Pakshirajan K., Pugazhenthi G., ,. J. Clean. Prod. 2018, 196, 1282— 1291
[9]	Bautista L. F., Morales G., Sanz R ., Chemosphere 2015, 136, 273— 280
[10]	Xu H., Wang C. P., Wang K. J., ,Chem. J. Chinese Universities, 2015,36( 2), 344— 348 doi: 10.7503/cjcu20140727
	(徐恒, 汪翠萍, 王凯军. 高等学校化学学报, 2015, 36(2), 344— 348) doi: 10.7503/cjcu20140727
[11]	Fu R. B., Yang L. Q., Feng L. Y., Guo W., ,Chem. J. Chinese Universities, 2014,35( 4), 825— 830 doi: 10.7503/cjcu20130806
	(付融冰, 杨兰琴, 冯雷雨, 郭伟.高等学校化学学报, 2014, 35(4), 825— 830) doi: 10.7503/cjcu20130806
[12]	Liu H., Logan B.E., ,. Environ. Sci. Technol. 2004, 38( 14), 4040— 4046
[13]	Cheng S., Liu H., Logan B. E., ,. Electrochem. Commun. 2006, 8( 3), 489— 494
[14]	Slate A. J., Whitehead K. A., Brownson D. A. C., Banks C. E., ,. Renew. Sust. Energ. Rev. 2019, 101, 60— 81
[15]	Wang L., You L., Zhang J., Yang T., Zhang W., Zhang Z., Liu P., Wu S., Zhao F., Ma J., ,. J. Hazard. Mater. 2018, 360, 402— 411
[16]	Li X., Wang X., Wan L., Zhang Y., Li N., Li D., Zhou Q., ,. J. Chem. Technol. Biot. 2016, 91, 267— 275
[17]	Malvankar N. S., Tuominen M. T., Lovley D. R., ,. Energ. Environ. Sci. 2012, 5, 5790— 5797
[18]	Logan B. E., ,. Nat. Rev. Microbiol. 2009, 7, 375— 381
[19]	Zhang T., Gannon S. M., Nevin K. P., Franks A. E., Lovley D. R., ,. Environ. Microbiol. 2010, 12, 1011— 1020
[20]	Morris J. M., Jin S., Crimi B., Pruden A., ,. Chem. Eng. J. 2009, 146, 161— 167
[21]	Zhao X., Wang Y., Ye Z., Borthwick A. G. L., Ni J., ,. Process. Biochem. 2006, 41, 1475— 1483
[22]	Odebunmi E. O., Adeniyi S. A., ,. Bull. Chem. Soc. Ethiop. 2007, 21( 1), 135— 140
[23]	Xu N . N., Bioadsorption, Uptake and Biodegradation of Polycyclic Aromatic Hydrocarbons by pH Microbial Consortium, Ocean University of China, Qingdao, 2014
	( 徐娜娜 . pH混合菌对多环芳烃的吸附、摄取及生物降解, 青岛: 中国海洋大学, 2014)
[24]	Xu N., Bao M., Sun P., Li Y., ,. Bioresour. Technol. 2013, 149, 22— 30
[25]	Fang Y., Deng C., Chen J., Lu J., Chen S., Zhou S., ,. Bioresour. Technol. 2018, 266, 548— 554
[26]	Rabaey K., Boon N., Siciliano S. D., Verhaege M., Verstraete W., ,. Appl. Environ. Microbiol. 2004, 70, 5373— 5382
[27]	Zhang Y., Wen J., Chen X., Huang G., Xu Y., Yuan Y., Sun J., Li G., Ning X. A., Lu X., Wang Y ., Chemosphere 2018, 211, 202— 209
[28]	Wang M. C., Liu T. T., Zhang X. J., Wu D., Fan L. P., ,J. Fuel. Chem. Technol., 2018,46( 6), 762— 768
	(王美聪, 刘婷婷, 张学军, 吴丹, 樊立萍.燃料化学学报,2018,46(6), 762— 768)
[29]	Ou S. Q., Kashima H., Aaron D. S., Regan J. M., Matthew M. M., ,. J. Power Sources 2017, 347, 159— 169
[30]	Yu Y. Y., Wang G. Z., Wang Z. R., Ding J., ,Environ. Sci. Technol., 2017,40( 8), 128— 133
	(于洋洋, 王广智, 王卓然, 丁杰.环境科学与技术,2017,40(8), 128— 133)
[31]	Xiang Y., Liu G., Zhang R., Lu Y., Luo H., ,. Bioresour. Technol. 2017, 241, 821— 829
[32]	Xiang Y., Liu G., Zhang R., Lu Y., Luo H., ,. Bioresour. Technol. 2017, 233, 227— 235
[33]	Ishii S., Suzuki S., Yamanaka Y., Wu A., Nealson K. H., Bretschger O ., Bioelectrochemistry 2017, 117, 74— 82
[34]	Yu J., Cho S., Kim S., Cho H., Lee T., ,. Microbes. Environ. 2012, 27, 49— 53
[35]	Lee S. Y., Park J. H., Jang S. H., Nielsen L. K., Kim J., Jung K. S., ,. Biotechnol. Bioeng. 2008, 101, 209— 228
[36]	Chignell J. F., De Long S. K., Reardon K. F., ,. Biotechnol. Biofuels. 2018, 11, 121— 137
[37]	Miran W., Jang J., Nawaz M., Shahzad A., Lee D. S., ,. J. Hazard. Mater. 2018, 352, 70— 79
[38]	Lack J. G., Chaudhuri S. K., Chakraborty R., Achenbach L. A., Coates J. D., ,. Microb. Ecol. 2002, 43, 424— 431
[39]	Hua T., Li S. N., Di Z. H., Zhou B., Zeng W. L., Zhou Q. X., Li F. X., ,Biotechnol. Bull., 2018,34( 10), 1— 6
	(华涛, 李胜男, 邸志珲, 周博, 曾文炉, 周启星, 李凤祥.生物技术通报,2018,34(10), 1— 6)
[40]	Li X., Zhao Q., Wang X., Li Y., Zhou Q., ,. J. Hazard. Mater. 2018, 344, 23— 32