Preparation of Amine-modified Poly(γ-benzyl-L-glutamate) Guided Bone Regeneration Film†

doi:10.7503/cjcu20180354

Abstract

Abstract:

Guided bone regeneration film plays an important role in bone defect reconstruction. In this paper, poly(γ-benzyl-L-glutamate)(PBLG) was used as raw materials to construct dense films and porous films by solvent casting and particle leaching. And the bilayers guided bone regeneration membrane was constructed by ethanolamine amine membrane modification on the surface. The effect of different modification time on the hydrophilicity and mechanical properties of the films was investigated. The results show that with the increase of the molecular weight of PBLG, the mechanical strength of the film increases and the degradation rate is getting slower. Prolonging the time of amine modification can improve the hydrophilicity of the film and the degradation rate both in vivo and in vitro. Through cell experiments, it was found that the dense layer could effectively block the invasion of fibroblasts, and the porous layer can support adherence and spread of cells. Surface-modified PBLG-based materials were demonstrated to be useful for repair of bone defects in vivo by in vitro bioactivity evaluation. The guided bone regeneration membrane constructed has good mechanical properties, degradation performance, and a certain degree of fit with the tissue. And it could effectively inhibit the infiltration of fibroblasts. The membrane has potential application value.

Key words: Poly(γ-benzyl-L-glutamate)(PBLG);, Ethanolamine ammonolysis, Guided bone regeneration

CLC Number:

O631

TrendMD:

LU Jiawei,ZHANG Kunxi,YU Xi,YAN Shifeng,YIN Jingbo. Preparation of Amine-modified Poly(γ-benzyl-L-glutamate) Guided Bone Regeneration Film^†[J]. Chem. J. Chinese Universities, 2019, 40(3): 601.

Figures/Tables 16

Scheme 1 Fabrication of amine-modified PBLG guided bone regeneration filmAfter cell seeding, the barrier function and biocompatibility of the membranes were evaluated.

Scheme 2 Schematic diagram of barrier function characterization of membrane(A) PBLG-s-PHEG bilayer films; (B) PBLG-s-PHEG-SPM.

Table 1 Molecular weight and distribution of PBLG with different molar ratio of monomer(A) and initiator(I)

Entry	n(A)/n(I)	M_w	M_η	Polydispersity
1	50	11×10⁴	8×10⁴	2.449
2	100	25×10⁴	18×10⁴	1.742

Fig.1 1H NMR(A) and FTIR(B) spectra of PBLG(a) and PBLG-s-PHEG(b)

Fig.2 SEM images of the sarface(A1, B1) and cross-section(A2, B2) of PBLG-s-PHEG-SDM(A1, A2) and PBLG-s-PHEG-SPM(B1, B2)

Fig.3 SEM image of cross-section of PBLG-s-PHEG(A) and thickness(B) of PBLG-s-PHEG-SDM(a),PBLG-s-PAEG-SPM(b) and PBLG-s-PHEG(c)

Fig.4 Mechanical strength of PBLG-s-PHEG membranes with different Mη of PBLG and different reaction time(A) Tensile strength; (B) suture retention strength. Mη of PBLG: (C) 8×104; (D) 18×104.

Fig.5 Variation of the contact angle of the PBLG membranes with different Mη and reacton timeMη of PBLG: (A) 8×104; (B) 18×104.

Fig.6 Images of the model of bone defect and adhesion of bilayer membranes after surface hydroxyl modification(A1) Model of bone defect; (A2) PBLG bilayer membranes; (A3) PBLG-s-PHEG membranes.

Fig.7 In vitro degradation of PBLG bilayer membrances(a) and PBLG-s-PHEG membranes(b) with different reacting time and Mn of PBLG in PBS solutionMn of PBLG: (A) 8×104; (B) 18×104.

Fig.8 SEM images of PBLG-s-PHEG membranes in vitro degradation with PBLG-s-PHEG-SDM membrane(A1—A3) and PBLG-s-PHEG-SPM membrane(B1—B3) at 7 d(A1, B1), 84 d(A2, B2) and 126 d(A3, B3)

Fig.9 In vivo degradation of the PBLG-s-PHEG membranes with 7 d(A1—A3), 84 d(B1—B3) and 126 d(C1—C3)(A1—C1) Cross appearance images; (A2—C2) SEM images; (A3—C3) H&E images.

Fig.10 Degradation of mass change of the PBLG-s-PHEG member with Mη of PBLG of 8×104 and reaction time of 12 h in vivo

Fig.11 Images of L929 fibroblasts proliferate on guided bone regeneration membrane(A) L929 fibroblasts proliferate on the dense structure of the PBLG-s-PHEG bilayers film; (B) L929 fibroblasts proliferate on the porous structure of the PBLG-s-PHEG bilayer membranes; (C) L929 fibroblasts proliferate on PBLG-s-PHEG-SPM.

Fig.12 SEM images of the growth of HA on the surface of PBLG-s-PHEG bilayer membranes in SBF solutionTime/d: (A) 0; (B) 3; (C) 7; (D) 14.

Fig.13 Thermogravimetric curve of amine-modified PBLG bilayer membranes in SBF solution with different time

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