Preparation of MoS2 Hollow Nanospheres and Application in Ultrasensitive Electrochemical Biosensing Platform for Micro-RNA Detection†

doi:10.7503/cjcu20160488

Abstract

Abstract:

Molybdenum disulfide hollow sphere nanomaterial was synthesized through template-assisted method, the morphological structure was characterized by scanning electron microscopy(SEM), X-ray diffraction(XRD) and Raman spectroscopy. A novel microRNA electrochemical biosensor was constructed by immobilizing of aptamer on the electrode that was modified with Au nanoparticles and MoS₂. The electrochemical behavior of the modified electrode was tested by cyclic voltammograms, differential pulse voltammetric and electrochemical impedance. The results indicate that the as-prepared biosensor shows a good linear relationship between the current variation and logarithm of the miRNA concentration ranging from 1.0×10^-10 mol/L to 1.0×10^-16 mol/L. The detection limit was estimated to be 0.055×10^-16 mol/L. The constructed sensor shows good selectivity, high sensitivity, strong stability characteristics and has a broad application prospect.

Key words: Molybdenum disulfide hollowsphere, MicroRNA, Electrochemical biosensor, Signal amplification

CLC Number:

O657

TrendMD:

LIU Renzhi, LI Xiaoyan. Preparation of MoS₂ Hollow Nanospheres and Application in Ultrasensitive Electrochemical Biosensing Platform for Micro-RNA Detection^†[J]. Chem. J. Chinese Universities, 2017, 38(3): 383.

Figures/Tables 13

Scheme 1 Schematic illustration of preparation process of biosensor for miRNA detection based on hollow MoS2 sphere coupling with enzyme-amplification

Fig.1 SEM images of MnCO3(A, B), MnS@MoS2(C), intact MoS2 sphere(D), cracked MoS2 sphere(E) and the high-resolution MoS2 sphere(F)

Fig.2 Raman spectra of MnCO3(A), MnS@MoS2 and MoS2(B)

Fig.3 XRD patterns of MnCO3(A), MnS@MoS2 and MoS2(B)

Fig.4 CVs(A,B) and EIS(C,D) of different electrodes (A), (C) a. GCE; b. MoS2/GCE; c. AuNPs/MoS2/GCE; (B),(D) a. AuNPs/MoS2/GCE; b. capture probe/AuNPs/MoS2/GCE; c. MCH/capture probe/AuNPs/MoS2/GCE; d. miRNA/MCH/capture probe/AuNPs/MoS2/GCE; e. signal probe/miRNA/MCH/capture probe/AuNPs/MoS2/GCE; f. SA-ALP/signal probe/miRNA/MCH/Capture probe/AuNPs/MoS2/GCE.

Fig.5 Q-t curves(A) and Q-t1/2 curves(B) of the bare GCE(a) and AuNPs/MoS2/GCE(b) in PBS containing 0.1 mmol/L K3[Fe(CN)6] and 1.0 mol/L KCl

Fig.6 DPVs of SA-ALP/signal probe/miRNA/MCH/capture probe/AuNPs/GCE(a) and SA-ALP/signal probe/miRNA/MCH/capture probe/AuNPs/MoS2/GCE(b) in 10 mmol/L PBS(pH=7.0) containing 5 mmol/L TCEP and 2 mmol/L FCM

Fig.7 Experimental conditions optimization (A) Deposition time of AuNPs; (B) incubation time of target miRNA; (C) incubation time of signal probe.

Fig.8 Schematic diagram of enzyme signal amplification system

Fig.9 DPVs of various target miRNA concentrations c(miRNA)/(mol·L-1) from a to j: 0, 1.0×10-16, 1.0×10-15, 1.0×10-14, 5.0×10-13, 1.0×10-13, 5.0×10-12, 1.0×10-12, 1.0×10-11, 1.0×10-10. Inset: the calibration plots of the DPV current versus the logarithm concentration of target miRNA.

Table 1 Comparison between the as-prepared sensor and other sensors for miRNA detection

Electrode	Analytical technique	Linear range/(pmol·L^-1)	LOD/(fmol·L^-1)	Ref.
Os(bpy)₂(API)Cl-activated GO	Amperometric current-time curve	0.008—10	4	[30]
HRP/TMB/gold GCE	Amperometric current-time curve	0.01—1000	10	[31]
HRP/AuNPs/graphene	DPV	0.001—700	6	[32]
AuNPs/ALP/redox cycling	Amperometric current-time curve	0.001—5	3	[33]
QDs/RP/GODPS/GCE	Fluorescence dots	0.05—1	5	[34]
IgG-ALP/AuNPs/GCE	DPV	0.0005—0.5	0.4	[35]
SA-ALPs/gold GCE	Mperometric current-time curve	0.0005—1	0.2	[36]
AuNPs/MoS₂/GCE	DPV	0.0001—100	0.055	This work

Fig.10 Selectivity of the sensor hybridized to different miRNA sequences a. Non-complementary sequence; b. three-based mismatch sequence; c. single-based mismatch sequence; d. target miRNA.

Table 2 Result of detection of directly adding target miRNA in serum samples(n=3)

Added miRNA/(fmol·L^-1)	Found miRNA/(fmol·L^-1)	RSD^*(%)	Recovery(%)
10	9.1	5.2	91.0
100	98.6	3.8	98.6
1000	1054	2.7	105.4
10000	9784	2.8	97.8
100000	105248	4.6	105.3

References 36

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Preparation of MoS₂ Hollow Nanospheres and Application in Ultrasensitive Electrochemical Biosensing Platform for Micro-RNA Detection^†

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