Interaction Mechanism Between Synthetic Receptor and Model Phosphorylated Peptides†

doi:10.7503/cjcu20140436

Chem. J. Chinese Universities ›› 2015, Vol. 36 ›› Issue (1): 171.doi: 10.7503/cjcu20140436

• Physical Chemistry • Previous Articles Next Articles

Interaction Mechanism Between Synthetic Receptor and Model Phosphorylated Peptides^†

ZHANG Saihui, SHI Yantao, HAN Liang, LI Chuanguang, WANG Wei, YUAN Zhi^*()

Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Nankai University, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300071, China

Received:2014-05-08 Revised:2014-12-16 Online:2015-01-10 Published:2014-12-16
Contact: YUAN Zhi E-mail:zhiy@nankai.edu.cn
Supported by:
† Supported by the National Natural Science Foundation of China(Nos.21104034, 51273094) and the Program for Changjiang Scholars and Innovative Research Team of Ministry of Education, China(NoIRT1257)

Abstract

Abstract:

Study on interaction mechanism between synthetic receptor and phosphorylated peptides is of great importance for phophopeptide enrichment and early disease-detection. In this study, association between Zn²⁺-coordination type synthetic receptor and some model phosphorylated peptides was studied, the receptor contains Zn²⁺-dipicolylamine and guanidiniocarbonyl pyrrole tethered by hydrophobic spacer. Isothermal titration calorimetry was used to elucidate the association thermodynamics. The receptor displayed higher affinity to bis-phosphorylated peptides in comparison with mono-phosphorylated peptides, and the association constant was 10—40 times higher for bis-phosphorylated peptides than that of mono-phosphorylated peptides. Space between the two phosphate groups showed great influence on the association, i.e. association constant remar-kably decreased as the space increased. For mono-phosphorylated peptides, the position of phosphate in the sequence affected the affinity. The results are expected to provide insights into the principle for the design of receptor for peptides.

Key words: Synthetic receptor, Phosphorylated peptides, Isothermal titration calorimetry

CLC Number:

O642

TrendMD:

ZHANG Saihui, SHI Yantao, HAN Liang, LI Chuanguang, WANG Wei, YUAN Zhi. Interaction Mechanism Between Synthetic Receptor and Model Phosphorylated Peptides^†[J]. Chem. J. Chinese Universities, 2015, 36(1): 171.

Figures/Tables 5

References 23

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Peptide sequence^*	10^-4K/ (L·mol^-1)	10^-4ΔH/ (J·mol^-1)	ΔS/ (J·mol^-1·K^-1)
GGpSpSGGG	8.7±1.2	7.9±1.5	337.2±46.9
GGpSGpSGG	3.6±0.6	13.0±1.3	527.2±62.8
GGpSGGpSG	2.6±0.4	35.1±4.6	202.9±18.4

Peptide sequence^*	10^-4K/ (L·mol^-1)	10^-4ΔH/ (J·mol^-1)	ΔS/ (J·mol^-1·K^-1)
GGpSpSGGG	8.7±1.2	7.9±1.5	337.2±46.9
GGpSGpSGG	3.6±0.6	13.0±1.3	527.2±62.8
GGpSGGpSG	2.6±0.4	35.1±4.6	202.9±18.4

Peptide sequence	10^-3K/ (L·mol^-1)	10^-3ΔH/ (J·mol^-1)	ΔS/ (J·mol^-1·K^-1)
GGGGGpSG	2.4±0.3	3.5±2.1	76.1±5.8
GGGGpSGG	9.8±0.5	4.4±2.3	87.0±5.0
GGGpSGGG	4.6±0.5	0.9±0.1	73.2±1.3
GGpSGGGG	1.7±0.3	4.7±0.9	77.4±3.3

Peptide sequence	10^-3K/ (L·mol^-1)	10^-3ΔH/ (J·mol^-1)	ΔS/ (J·mol^-1·K^-1)
GGGGGpSG	2.4±0.3	3.5±2.1	76.1±5.8
GGGGpSGG	9.8±0.5	4.4±2.3	87.0±5.0
GGGpSGGG	4.6±0.5	0.9±0.1	73.2±1.3
GGpSGGGG	1.7±0.3	4.7±0.9	77.4±3.3

Peptide sequence	10^-3K/ (L·mol^-1)	10^-3ΔH/ (J·mol^-1)	ΔS/ (J·mol^-1·K^-1)
GGGGpSGG	9.8±0.5	4.2±2.1	87.0±5.0
GGGGpSFG	4.5±0.3	9.2±0.4	100.0±5.0
GGGFpSGG	4.7±0.5	10.9±0.4	106.7±8.8

Interaction Mechanism Between Synthetic Receptor and Model Phosphorylated Peptides^†

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