Low-background Electrochemical DNA Biosensor Using an Electrically Neutral Osmium Complex as Hybridization Indicator

doi:10.7503/cjcu20120705

Abstract

Abstract:

The interaction between an electrically neutral osmium complex Os(DPPZ)(PC)(H₂O) (DPPZ=dipyrido(3,2-a,2',3'-c)phenazine, PC=2,6-pyridine dicarboxylic acid) and DNA was investigated by UV absorption spectroscopy and electrochemical methods. The hypochromic and red-shift effects of the characteristic absorption peak of Os(DPPZ)(PC)(H₂O) after interaction with DNA suggested that the complex bound to DNA via an intercalative mode, which was testified by the positive shift of the formal potential of the complex in cyclic voltammetric experiments. When the complex was utilized as a hybridization indicator for the detection of CaMV35S promoter gene fragments, no electrochemical response was observed on the probe of DNA modified electrode. A sensitive electrochemical signal, however, was obtained on the hybridized electrodes, suggesting that the DNA biosensor possessed a high signal-to-noise ratio. The complementary target sequence could be quantified over the range from 8.0?0^-10 mol/L to 2.8?0^-9 mol/L. The hybridization specificity experiments further indicated that the biosensor could well discriminate the complementary sequences from the base-mismatched and the non-complementary sequences.

Key words: Electrically neutral osmium complex, Hybridization indicator, Electrochemical DNA biosensor, Intercalation

CLC Number:

O657.1
O626

TrendMD:

DING Ying-Tao, WANG Li-Heng, LI Fu-Xiao, GAO Feng, GAO Fei, WANG Qing-Xiang. Low-background Electrochemical DNA Biosensor Using an Electrically Neutral Osmium Complex as Hybridization Indicator[J]. Chem. J. Chinese Universities, 2013, 34(4): 823.

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