Interaction Between Methylene Blue and ctDNA by Time-resolved Spectroscopy

Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (8): 1854.

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Interaction Between Methylene Blue and ctDNA by Time-resolved Spectroscopy

LIU Tao, ZHANG Chong-Lei, CHEN Ping, TANG Guo-Qing, LIN Lie*

Key Laboratory of Optoelectronic Information Science and Technology, Ministry of Education, Institute of Modern Optics, Nankai University, Tianjin 300071, China

Received:2010-09-14 Revised:2010-11-26 Online:2011-08-10 Published:2011-07-19
Contact: LIN Lie E-mail:linlie@nankai.edu.cn
Supported by:
国家自然科学基金(批准号: 60508004, 60778043)和教育部博士学科点基金(批准号: 20060055024)资助.

Abstract

Abstract: In this paper, the singlet oxygen（1O2） kinetics process of MB-ctDNA mixed solutions in D2O was studied by time-resolved spectroscopy, which helped to further investigate the interaction of MB and DNA. The results showed that 1O2 phosphorescence kinetic curves of MB-ctDNA solutions at low concentrations ctDNA and high concentrations ctDNA had obvious differences, these differences were ascribed to change of MB and DNA binding mode and interaction mechanism. At low ctDNA concentration, ionic conjugate formed between MB molecules and ctDNA, significant hypochromic effect was observed in MB absorption band, and 1O2 production dropped sharply with ctDNA concentration increasing, but there were no obvious interaction between ctDNA and 1O2; at high ctDNA concentration, interaction mode between MB molecules and ctDNA changed to intercalative binding, energy transfer between the excited state MB molecules and ctDNA as well as the effect of medium viscosity changed property of the 1O2 dynamics, which greatly decreased the production of 1O2, but ctDNA did not quench the 1O2 apparently. These results indicated that type II photosensitization of 1O2 damaging DNA was not major mechanism of PDT action in MB-DNA solutions.

Key words: Time-resolved spectroscopy; , Singlet oxygen kinetics; , Methylene blue; , ctDNA

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LIU Tao, ZHANG Chong-Lei, CHEN Ping, TANG Guo-Qing, LIN Lie*. Interaction Between Methylene Blue and ctDNA by Time-resolved Spectroscopy[J]. Chem. J. Chinese Universities, 2011, 32(8): 1854.

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Interaction Between Methylene Blue and ctDNA by Time-resolved Spectroscopy

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