CJCU

Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (10): 2274.

• Preface • Previous Articles     Next Articles

Detection of E. coli O157∶H7 by Fluorescent Silica Nanoparticles and SYBR Green Ⅰ Based Two\|color Microscopic Imaging Technique

ZHOU Li-Xia1,2,4, HE Ding-Geng2,3,4, HE Xiao-Xiao1,2,4*, SHI Hui2,3,4, WANG Ke-Min1,2,3,4*, CAO Jie2,3,4   

  1. 1. College of Biology,
    2. State Key Laboratory of Chemo/Biosensing and Chemometrics,
    3. College of Chemistry and Chemical Engineering,
    4. Key Laboratory for Bio\|Nanotechnology and Molecule Engineering of Hunan Province, Hunan University, Changsha 410082, China
  • Received:2010-11-29 Revised:2011-01-09 Online:2011-10-10 Published:2011-09-11
  • Contact: WANG Ke-Min;HE Xiao-Xiao E-mail:kmwang@hnu.cn;xiaoxiaohe@hnu.cn
  • Supported by:

    科技部国际合作专项(批准号: 2010DFB30300)、国家“九七三”计划项目(批准号: 2002CB513100-10)、国家自然科学基金(批准号: 90606003, 20775021)和湖南省自然科学基金创新研究群体(批准号： 10JJ7002)资助.

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Abstract: A novel two-color immunofluorescent microscopic imaging technique for the rapid and sensitive detection of E. coli O157∶H7 was developed by fluorescent silica nanoparticles and SYBR Green Ⅰ. Based on antigen-antibody interaction, the anti-E. coli O157∶H7 antibody conjugated RuBpy-doped silica nanoparticles were used to recognize E. coli O157∶H7 specifically. The bacteria sample was then stained with a nucleic acid dye SYBR Green I and imaged with confocal microscope. The separation-free detection of E. coli O157∶H7 was successfully carried out in buffer and bacterial mixture, respectively. The whole detection process could be finished within 3 h, and the detection limit for E. coli O157∶H7 was 2.6×103Cell/mL. Considering the antibodies for various bacteria, this proposed method might be promising for rapid and sensitive detection of other bacteria.

Key words: Fluorescent silica nanoparticle, SYBR Green Ⅰ, E. coli O157∶H7, Two-color microscopic imaging

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