HPLC-ESI-MS Analysis of Ginsenosides and Antioxidant Activity During Combination of Ginseng with Aconitum Carmichaeli and Coptis Chinensis

Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (7): 1332.

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HPLC-ESI-MS Analysis of Ginsenosides and Antioxidant Activity During Combination of Ginseng with Aconitum Carmichaeli and Coptis Chinensis

DU Qin-Qin^1,2, ZHANG Xu¹, SONG Feng-Rui¹, LIU Zhi-Qiang¹, LIU Shu-Ying^1*

1. Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun 130022, China;
2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Received:2009-12-10 Online:2010-07-10 Published:2010-07-10
Contact: LIU Shu-Ying. E-mail: mslab@ciac.jl.cn
Supported by:
国家支撑计划(批准号: 2007BAI38B04-03)和吉林省科技发展重大及重点项目(批准号: 20086043, 20090908)资助.

Abstract

Abstract:

Eight ginsenosides in ginseng, uncombined and combined with Aconitum carmichaeli or Coptis chinensis, have been investigated by high performance liquid chromatography(HPLC) and electrospray ionization-mass spectrometry(ESI-MS). The experimental results demonstrated that the contents of ginsenosides decreased in the decoction of ginseng with Aconitum carmichaeli or Coptis chinensis, and the stripping of ginsenosides was stained during the combination. Furthermore, the antioxidant activity of n-butanol extract in compatible decoction was not only better than the single decoction of ginseng, but also higher than the mixture of the single decoction of ginseng, Aconitum carmichaeli and Coptis chinensis, while the variation of water extract was different, which was determined by ferric reducing antioxidant power(FRAP) method. That might be related to the interaction during the decoction process.

Key words: Ginseng; Aconitum carmichaeli; Coptis chinensis; HPLC-ESI-MS; Anti-oxidation

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DU Qin-Qin, ZHANG Xu, SONG Feng-Rui, LIU Zhi-Qiang, LIU Shu-Ying*. HPLC-ESI-MS Analysis of Ginsenosides and Antioxidant Activity During Combination of Ginseng with Aconitum Carmichaeli and Coptis Chinensis[J]. Chem. J. Chinese Universities, 2010, 31(7): 1332.

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HPLC-ESI-MS Analysis of Ginsenosides and Antioxidant Activity During Combination of Ginseng with Aconitum Carmichaeli and Coptis Chinensis

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