Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (7): 1322.

• Articles • Previous Articles     Next Articles

Novel Molecular Beacon with High Selectivity Based on Locked Nucleic Acids

WANG Qing, CUI Liang, YANG Xiao-Hai, WANG Ke-Min*, HE Lei-Liang   

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Biomedical Engineering Center, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China
  • Received:2009-12-28 Online:2010-07-10 Published:2010-07-10
  • Contact: WANG Ke-Min. E-mail: kmwang@hnu.cn
  • Supported by:

    国家“八六三”计划项目(批准号: 2007AA022007)、国家自然科学基金(批准号: 90606003, 20805012)、湖南省杰出青年科学基金(批准号: 08JJ1002)和湖南省高等学校青年骨干教师培养对象基金(2009年度)资助.

Abstract:

A novel kind of molecular beacon which was modified using locked nucleic acids at mismatch sites was reported. Hybridization kinetics of different molecular beacons with cDNA and single-base mismatch DNA, and melting temperature were investigated using fluorescence spectroscopy. The results showed that molecular beacon with only the mismatch site replaced with LNA had the highest single-base mismatch discrimination capability. Such novel molecular beacon could not only improve effectively single-base mismatch recognition ability, but also enhance the sensitivity. Probably, it was resulted by the high-affinity of locked nucleic acid. Therefore, it was expected to develop a common method for improving the single-base mismatch recognition ability.

Key words: Molecular beacon; Locked nucleic acid; Selectivity

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