Prefractionation Method to Separate Proteomic Proteins into Multigroups by One-step Extraction

Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (7): 1314.

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Prefractionation Method to Separate Proteomic Proteins into Multigroups by One-step Extraction

BAI Hai-Xin^1*, LIU Xiao-Hua¹, YANG Fan², YANG Xiu-Rong², WANG Er-Kang^2*

1. Department of Applied Chemistry, College of Sciences, Henan Agricultural University, Zhengzhou 450002, China;
2. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Received:2009-12-28 Online:2010-07-10 Published:2010-07-10
Contact: WANG Er-Kang. E-mail: ekwang@ciac.jl.cn; BAI Hai-Xin. E-mail: haixin-bai@yahoo.com.cn
Supported by:
国家自然科学基金(批准号: 20735003)和电分析化学国家重点实验室基金(批准号: SKLEAC2010011)资助.

Abstract

Abstract:

For the first time, an aqueous two-phase system(ATPS) composed of n-propanol, NaCl and water was applied in prefractionating proteomic proteins. In this work, ethanol, n-propanol and several commonly used inorganic salts were attempted to develop the ATPS. The ATPS composed of n-propanol, NaCl and water was developed finally. Subsequently, the effects of pH, salt concentration, n-propanol volume and protein concentration on the ATPS′ separation effectiveness for proteins were studied. Protein samples were prefractionated by the ATPS and then analyzed by gel electrophoresis. The results proved that the prefractionation method could separate proteomic proteins into three groups by one-step extraction, and there was no need for separating target proteins from the phase-forming reagents prior to gel electrophoresis analysis. Simplicity, rapidity and low cost are the highlight attractiveness of the prefractionation method. It also has advantages of biocompability, potential for continuous operation, no requirement for sophisticated instrumentations and being easy or no need for separating the target proteins from phase-forming reagents prior to electrophoresis analysis. The prefractionation method has great significance in proteomics and methodology.

Key words: Proteomic prefractionation method; Aqueous two-phase system; Extraction; Separation

TrendMD:

BAI Hai-Xin*, LIU Xiao-Hua, YANG Fan, YANG Xiu-Rong, WANG Er-Kang*. Prefractionation Method to Separate Proteomic Proteins into Multigroups by One-step Extraction[J]. Chem. J. Chinese Universities, 2010, 31(7): 1314.

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Prefractionation Method to Separate Proteomic Proteins into Multigroups by One-step Extraction

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