Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (5): 927.

• Articles • Previous Articles     Next Articles

Metabonomic Study of Aristolochic Acid I-induced Acute Renal Toxicity Urine at Female and Male C57BL/6J Mice Based on 1H NMR

LIU Xia1, XIAO Ying1, GAO Hong-Chang2*, REN Jin1*, LIN Dong-Hai1*   

  1. 1. Analytical Chemistry Laboratory, State Key laboratory of New Drug Research, Centre for Drug Safety and Evaluation Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
    2. Analysis and Testing Center, Pharmacy School, Wenzhou Medical College, Wenzhou 325035, China

  • Received:2009-05-14 Online:2010-05-10 Published:2010-05-10
  • Contact: LIN Dong-Hai. E-mail: dhlin@mail.shcnc.ac.cn; REN Jin. E-mail: jren@mail.shcnc.ac.cn; GAO Hong-Chang. E-mail: gaohc27@gmail.com
  • Supported by:

    国家自然科学基金(批准号: 20705037)、温州医学院科研启动经费(批准号: QTJ08008)、中国科学院知识创新工程青年人才领域前沿和重要方向项目(批准号: SIMM0709QN-07, KSCX2-YW-R-118)资助.

Abstract:

The present work illustrates the metabolic changes of aristolochic acid I-induced acute renal toxicity urine at male and female C57BL/6J mice, which would help to understand the mechanism of renal toxicity and the differences in the metabolism at female and male mice after AAI treatment. The results showed that AAI nephrotoxicity mainly resulted from AAI inhibited the tricaboxylic acid cycle and energy metabolism,damaged the ecology of intestinal flora, changed the osmotic pressure inside and outside the kidney cells, depressed creatinine clearance rate and caused the injury of renal tubular and glomerular. Metabolic pattern analysis also showed that male mice were more sensitive to AAI renal toxicity than the female. These results suggested that urine 1H NMR-based metabonomics probably provides a novel insight into the study of drug toxicity mechanism and the toxicity gender differences.

Key words: Aristolochic acid; Renal toxicity; Metabonomics; NMR; Toxicity gender difference

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