Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (5): 1060.

• Articles • Previous Articles    

Effect of Chitosan Modification on the Behavior of the Immobilized Redox Enzyme on Nanofibrous Membranes

HUANG Fu, WANG Zhen-Gang, WAN Ling-Shu, HUANG Xiao-Jun, XU Zhi-Kang*   

  1. Key Laboratory of Macromolecular Synthesis and Functionalization, Ministry of Education, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China
  • Received:2009-08-04 Online:2010-05-10 Published:2010-05-10
  • Contact: XU Zhi-Kang. E-mail: xuzk@zju.edu.cn
  • Supported by:

    国家“八六三”计划项目(批准号: 2007AA10Z301)和国家杰出青年基金(批准号: 50625309)资助.

Abstract:

Poly(acrylonitrile-co-acrylic acid)(PANCAA) nanofibrous membranes were fabricated by electrospinning process. The electrospinning of PANCAA was optimized and the nanofibrous membranes were characterized using field emission scanning electron microscopy(FESEM). Then, chitosan was tethered on the surfaces of the PANCAA nanofibrous membranes in the presence of EDC/NHS. The chemical changes of the membrane surface were characterized by attenuated total reflectance/Fourier transform infrared spectroscopy(ATR/FTIR) and water contact angle measurement. Catalase was immobilized onto the modified nanofibrous membranes using glutaraldehyde(GA) as coupling agent. The enzyme activity and stability were compared with those of the nascent nanofibrous membrane. The max activity was (1011.6±2.1) U/mg under 25 mg/mL chitosan and 2.5% glutaraldehyde for immobilized enzyme. It was found that the activity retention of the immobilized catalase was enhanced by 41.7% and the stability was also enhanced.

Key words: Nanofibrous membrane; Surface modification; Enzyme immobilization; Catalase; Chitosan

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