Effect of 17-β-Estradiol on Hypoxia-reoxygenation-induced NF-κB Activity, ICAM-1 and VCAM-1 Expression in Cardiac Myocytes

Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (4): 746.

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Effect of 17-β-Estradiol on Hypoxia-reoxygenation-induced NF-κB Activity, ICAM-1 and VCAM-1 Expression in Cardiac Myocytes

HUO Hong-Liang, AN Shang-Yu, ZHONG Shi-Gang, ZENG Qing-Hua*

School of Life Sciences, Northeast Normal University, Changchun 130024, China

Received:2009-02-26 Online:2010-04-10 Published:2010-04-10
Contact: ZENG Qing-Hua. E-mail: zengqh707@nenu.edu.cn
Supported by:
国家自然科学基金(批准号: 30870910)资助.

Abstract

Abstract:

Transcription factor(NF)-κB plays an important role during hypoxia-reoxygenation in cardiac myocytes. In previous studies, it has been proved that NF-κB can bind to the specific sequences located in the promoters of ICAM-1 and VCAM-1, ediate the expression of tow adhesion molecules, and cause the cell inflammatory response and apoptosis. In order to analyze the anti-inflammation function of 17-β-estradiol during hypoxia-reoxygenation, we established the hypoxia-reoxygenation model in cardiac myocytes which were isolated from neonatal rat. And then, we detected the effect of 17-β-estradiol on activation of NF-κB p65 and studied the estrogen on how to adjust the level of ICAM-1 and VCAM-1 by Flow Cytometry, Western Blot, RT-PCR and Elisa technology. The results show that the NF-κB pathway is activated during hypoxia-reoxygenation, and 17-β-estradiol inhibits this activation significantly. 17-β-Estradiol also inhibits the expression of ICAM-1 and VCAM-1 by both NF-κB-dependent and NF-κB-independent pathway. Taken together, all results suggested that anti-inflammatory effect of 17-β-estradiol during myocardial hypoxia-reoxygenation is achieved through multichannels.

Key words: 17-β-Estradiol; Cardiac myocytes; Hypoxia-reoxygenation; NF-κB; Adhesion molecule

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HUO Hong-Liang, AN Shang-Yu, ZHONG Shi-Gang, ZENG Qing-Hua*. Effect of 17-β-Estradiol on Hypoxia-reoxygenation-induced NF-κB Activity, ICAM-1 and VCAM-1 Expression in Cardiac Myocytes[J]. Chem. J. Chinese Universities, 2010, 31(4): 746.

References

[1]LI Ying(李莹), WANG Yi(王毅), QU Hai-Bin(瞿海斌), et al.. Chem. J. Chinese Universities(高等学校化学学报)[J], 2008, 29(3): 515—518
[2]CHEN Zhan-Guo(陈战国), ZHAO Hai-Xia(赵海霞), WEI Jun-Fa(魏俊发), et al.. Chem. J. Chinese Universities(高等学校化学学报)[J], 2009, 30(1): 82—89
[3]Bebo B. F., Jr Fyfe-Johnson A., Adlard K., et al.. J. Immunol.[J], 2001, 166(3): 2080—2089
[4]Dubal D. B., Zhu H., Yu J., et al.. Proc. Natl. Acad. Sci. USA[J], 2001, 98(4): 1952—1957
[5]Ito. A., Bebo B. F. J., Matejuk A., et al.. J. Immunol.[J], 2001, 167(1): 529—542
[6]Serena G., Clara M., Adriana M., et al.. Molecular and Cellular Biology.[J], 2005, 25(8): 2957—2968
[7]Dean N. M., McKay R., Condon T. P., et al.. J. Biol. Chem.[J], 1994, 269(23): 16416—16424
[8]Bouillon M., Fortier M. A., Boulianne R., et al.. J. Cancer[J], 1992, 50(2): 281—288
[9]Pober J. S., Slowik M. R., De Luca L. G., et al.. J. Immunol.[J], 1993, 150(11): 5114—5123
[10]Renkonen R., Mennander A., Ustinov J., et al.. Int. Immunol.[J], 1990, 2(8): 719—724
[11]Wahl. C., Liptay S., Adler G., et al.. J. Clin. Investig.[J], 1998, 101(5): 1163—1174
[12]Yamamoto Y., Yin M. J., Lin K. M., et al.. J. Biol. Chem.[J], 1999, 274(38): 27307—27314
[13]Yin M. J., Yamamoto Y., Gaynor R. B.. Nature[J], 1998, 396: 77—80
[14]Nissen R. M., Yamamoto K. R.. Genes Dev.[J], 2000, 14(18): 2314—2329
[15]Ricote M., Li A. C., Willson T. M., et al.. Nature[J], 1998, 391(1): 79—82
[16]Auphan N., DiDonato J., Rosette C., et al.. Science[J], 1995, 270(5234): 286—290
[17]Kacimi R., Joel K. S., Koudssi F.. Circ. Res.[J], 1998, 82(5): 576—586
[18]Patten R. D., Pourati I., Aronovitz M. J., et al.. Circ. Res.[J], 2004, 95(7): 692—699
[19]Kousteni S., Bellido T., Plotkin L. I., et al.. Cell[J], 2001, 104(5): 719—730

Effect of 17-β-Estradiol on Hypoxia-reoxygenation-induced NF-κB Activity, ICAM-1 and VCAM-1 Expression in Cardiac Myocytes

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