Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (4): 736.

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Proteomic Analysis of Fear Memory Related Proteins in Mice

ZHENG Jun-Fang, LIU Hua, XIONG Ying, WANG Xiao-Zhu, HE Jun-Qi*   

  1. Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing 100069, China
  • Received:2009-07-01 Online:2010-04-10 Published:2010-04-10
  • Contact: HE Jun-Qi. E-mail: jq_he@ccmu.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 30772573, 30900247)、北京市科技新星计划B类项目(批准号: 2008B58)、教育部新世纪优秀人才项目(批准号: NCET-06-0184)、北京市优秀人才培养项目(批准号: 20071D0501800253)和首都医科大学科研基金(批准号: 2008ZR01)资助.

Abstract:

To investigate proteins related with fear and emotional memory, hippocampal proteins from CD1 and C57BL/6J mice after fear conditioning experiment were extracted and subjected to 2D gel electrophoresis. Protein quantification was analyzed and the differentially expressed spots were identified using mass spectrometry and MASCOT database searching. Hippocampal proteins from CD1 and C57BL/6J mice after fear conditioning experiment were significantly different. Twenty-nine proteins(Thirty-one spots) are closely related with fear memory formation. Expression levels of 7 spots downregulated significantly; expression levels of 24 spots increased significantly. These identified proteins could be devided into six categories according to their functions: (1) energy metabolism and mitochondrion function; (2) neurogenesis; (3) signal transduction; (4) cytoskeleton; (5) amino acid metabolism and protein degradation; (6) chaperone. These proteins improved insights into the fear mechanism and provided new targets for the study and treatment of cognitive diseases.

Key words: Fear conditioning; Hippocampus; Proteomics; Mass spectrometry

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