Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (4): 667.

• Articles • Previous Articles     Next Articles

On-chip Temperature Gradient Capillary Electrophoresis with Thermal Resistance Gradient Heating System for DNA Mutation Detection

XU Zhang-Run1*, LI Na1, ZHANG Hui-Dan2, FAN Xiao-Feng1, FANG Jin2*   

  1. 1. Research Center for Analytical Sciences, Northeastern University, Shenyang 110004, China;
    2. Department of Cell Biology, Key Lab of Cell Biology of Ministry of Public Health, Key Laboratory of Medical Cell Biology of Ministry of Education, China Medical University, Shenyang 110001, China
  • Received:2009-11-16 Online:2010-04-10 Published:2010-04-10
  • Contact: XU Zhang-Run. E-mail: xuzr@mail.neu.edu.cn; FANG Jin. E-mail: jfang61@netease.com
  • Supported by:

    国家“九七三”计划项目(批准号: 2007CB714503)、国家自然科学基金(批准号: 20675012)和辽宁省教育厅重点实验室支持计划(批准号: 20060907)资助.

Abstract:

A simple and reliable on-chip spatial temperature gradient capillary electrophoresis was developed for DNA mutation detection using a thermal resistance gradient heating system. Polydimethylsiloxane(PDMS) slab with thermal resistance varying in gradient was made, and continuous temperature gradient was formed on slab surface using a single thermostated copper heater block owing to heat exchange influenced by varied thermal resistance. Different temperature gradients were produced by regulating the thickness of PDMS slab, and the temperature gradients were stable and uniform within 6 h. Utilizing the heating system, 209 bp DNA mutation standard samples were separated within a wide temperature range of 10 ℃ on a glass microchip with an assay time of 8.3 min. Effectiveness of the system was demonstrated by the successful detection of K-ras gene mutations in paraffin tissue sections from 3 colorectal cancer patients.

Key words: Microfluidic chip; Capillary electrophoresis; Temperature gradient; DNA mutation

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