Stress Proteins of Gill Tissue in Patinopecten yessoensis Exposed to Cadmium Salt

Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (3): 507.

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Stress Proteins of Gill Tissue in Patinopecten yessoensis Exposed to Cadmium Salt

FANG Cai-Wang¹, HUANG Qing-Yu^1,2, LING Xue-Ping^1,3, KE Cai-Huan², HUANG He-Qing^1,2,3*

1. Key Laboratory of Cell Biology and Tumor Cell Engineering, Ministry of Education, School of Life Sciences,
2. State Key Laboratory of Marine Environmental Science,
3. Department of Chemical Biology, College of Chemistry & Chemical Engineering, Xiamen University, Xiamen 361005, China

Received:2009-05-08 Online:2010-03-10 Published:2010-03-10
Contact: HUANG He-Qing
Supported by:
国家自然科学基金(批准号: 04776060)和国家“九七三”计划项目(批准号: 2010CB12640)资助.

Abstract

Abstract:

The organelle ultrastructure in gill tissue was observed by transmission electron microscope(TEM) in Patinopecten yessoensis(PY), finding the pathological changes of gill filaments, nucleus and mitochondria in the tissue exposed to CdCl₂(10 mg/L). The proteome of gill tissue in PY was perfectly separated by two-dimensional polyacrylamide gel electrophoresis(2D-PAGE), obtaining approximately 800 protein spots, and selecting 37 differential spots intimidated with cadmium salt in the gel. In addition, these differential proteins were identified by both matrix-assisted laser desorption/ionization-time of flight mass spectrometry(MALDI-TOF MS) and database searching. The results showed that 7 differential proteins tightly connected with the cadmium toxicity were considered to be up-regulated proteins such as heat shock protein 70 and β-amylase, and down-regulated proteins such as tropomyosin, actin and calcium activated nucleotidase 1. Moreover, transcriptional regulator Crp/Fnr family showed low expression, while ABC transporter showed high expression. We suggest that these differential proteins in part have strong potentials to be utilized as protein biomarkers for monitoring the pollution level of cadmium continuously and evaluating its risk to organisms.

Key words: Proteomics; Patinopecten yessoensis; Gill; Cadmium stress; Stress protein

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FANG Cai-Wang, HUANG Qing-Yu, LING Xue-Ping, KE Cai-Huan, HUANG He-Qing. Stress Proteins of Gill Tissue in Patinopecten yessoensis Exposed to Cadmium Salt[J]. Chem. J. Chinese Universities, 2010, 31(3): 507.

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Stress Proteins of Gill Tissue in Patinopecten yessoensis Exposed to Cadmium Salt

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