Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (2): 269.

• Articles • Previous Articles     Next Articles

MALDI-TOF-MS for Lipidomics Analysis——Potential Biomarker for Cadmium Effect on Crucian Carp(Carassius auratus)

LI Guo-Chen1 , WANG Yan-Hong1*, WU Ren-An2, WANG Shi-Cheng1   

  1. 1. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;
    2. Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
  • Received:2009-06-24 Online:2010-02-10 Published:2010-02-10
  • Contact: WANG Yan-Hong. E-mail: wangyh@iae.ac.cn
  • Supported by:

    中国科学院知识创新工程重要方向项目(批准号: KSCX2-YW-N-035)和沈阳市科技计划项目(批准号: 2007GX-49)资助.

Abstract:

The biochemical effects of cadmium(Cd) on Crucian Carp was studied by MALDI-TOF-MS-based lipidomics. Crucian Carps were exposed to water with 500 μg/L Cd2+ for 0, 10, 20 d, respectively. Muscle samples from Cd-treated fishes were analyzed either by Atomic absorption spectrophotometer(AAS) for Cd contents, or analyzed by HPLC and MALDI-TOF-MS for phospholipids. All MALDI-TOF-MS spectra data were analyzed by pattern recognition using partial least square-discriminant analysis(PLS-DA). During the whole exposure period, Cd contents in muscle kept increasing, while phosphatidylcholine(PC) contents after 10 d exposure were significantly lower than that of normal level(no  exposure)(p<0.05), and the PC contents after 20 d exposure were nor significantly different from the normal level. The three groups were discriminated successfullyby PLS-DA, which suggested that lipidomics studies could better reflect the biochemical effects of Cd. PC could be the biomarker indicating the toxicity of Cd to Crucian Carp. Offline combination of HPLC and MALDI-TOF-MS could not only give the quantify PC, but also isolate and purify PC substraction, hereby it was applicable for lipidomics analysis.

Key words: Cadmium; Phospholipids; Biomarker; Lipidomics; MALDI-TOF-MS

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