Metabonomics Research of the Influence of Melamine to the Urine Metabolism of the Children Based on UPLC/TOF-MS

Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (1): 57.

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Metabonomics Research of the Influence of Melamine to the Urine Metabolism of the Children Based on UPLC/TOF-MS

WANG Yong^1,2, JIANG Zhi-Ting^1,2, LIANG Qiong-Lin², WANG Yi-Ming², WANG Mei³, LUO Guo-An^1,2*

1. School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China;
2. Department of Chemistry, Tsinghua University, Beijing 100084, China;
3. Emergency Department, Children Hospital of Changchun, Changchun 130051, China

Received:2009-03-30 Online:2010-01-10 Published:2010-01-10
Contact: LUO Guo-An. E-mail: luoga@tsinghua.edu.cn

Abstract

Abstract:

The metabonomics method was employed to investigate the possible influence of melamine to the urine metabolism of the children. Urine samples of 7 melamine affected children and 570 healthy children were collected from the Children Hospital of Changchun. The profiles of the collecteed urine samples were obtained with ultra performance liquid chromatography coupled with time-of-flight mass spectrometry(UPLC/TOF-MS). The data were firstly processed with the software MarkerLynx, then analyzed with principle component analysis(PCA) and partial least squares discriminant analysis(PLS-DA). The potential biomarkers were screened out according to the VIP(variable importance in projection) value and identified with i-fit and database. Most of the metabolites of significant intergroup differences were finally found to be in the tricarboxylic acid cycle. According to the biological functions of the obtained potential biomarkers, it was deduced that the citric acid metabolism was disturbed by melamine through its kidney injury effect. The results suggest that the metabonomics method can be utilized to investigation of the influence of melamine to the metabolism as well as the noninvasive examination of the melamine caused kidney injury.

Key words: Metabonomics; UPLC/TOF-MS; Melamine; Kidney injury; Citric acid metabolism

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WANG Yong, JIANG Zhi-Ting, LIANG Qiong-Lin, WANG Yi-Ming, WANG Mei, .... Metabonomics Research of the Influence of Melamine to the Urine Metabolism of the Children Based on UPLC/TOF-MS[J]. Chem. J. Chinese Universities, 2010, 31(1): 57.

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Metabonomics Research of the Influence of Melamine to the Urine Metabolism of the Children Based on UPLC/TOF-MS

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