Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (1): 11.

• Articles • Previous Articles     Next Articles

α-Chymotrypsin Immobilization on the Surface of Phase-transferred Fe3O4 Nanoparticles

WU Xia1,2, HAN Yu-Shun1,2, CAO Min-Hua1, HU Chang-Wen1,2, REN Ling1*, GE Guang-Lu2*   

  1. 1. College of Science, Beijing Institute of Technology, Beijing 100081, China;
    2. National Center for Nanoscience and Technology, Beijing 100190, China
  • Received:2009-07-03 Online:2010-01-10 Published:2010-01-10
  • Contact: REN Ling, E-mail: rl@bit.edu.cn; GE Guang-Lu, E-mail: gegl@nanoctr.cn
  • Supported by:

    国家自然科学基金(批准号: 20871016, 20673031)和中国科学院知识创新工程重点方向项目(批准号: KSCX2-YW-G-019)资助.

Abstract:

The oil-soluble magnetic Fe3O4 nanoparticles with uniform size and morphology were synthesized via thermal decomposition of Fe(acac)3 precursor. They were successfully transferred into aqueous phase through the ligand-exchange reaction with water-soluble PEG diacid molecule, forming water-soluble, stable, nontoxic, and magnetic composite with surface functional group. TEM, M-H curve, FTIR and TGA were used to characterize the as-synthesized composite and demonstrated that the nanoparticles maintained their superparamagnetism and biocompatibility. α-Chymotrypsin(α-ChT) was immobilized on the surface of the water-soluble nanoparticles. The immobilized α-ChT exhibited better thermal stability and high activity after repeated usage.

Key words: Magnetic Fe3O4 nanoparticle; Surface ligand exchange; Immobilized enzyme

TrendMD: