Abstract: The buccal ganglion(BG) was symmetrically divided into two sub-BG(SBG) in Aplysia(Notarcus leachii cirrosus Stimpson, NLCS) with reference to its ganglion shape, called left SBG(LSBG) and right SBG(RSBG). The whole proteins both LSBG and RSBG were optimally separated via two-dimensional polyacry-lamide gel electrophoresis(2D-PAGE), respectively. The differential proteins were further selected and identified by proteomic and comparison database techniques. The experimental results indicate that these differential proteins between LSBG and RSBG are identified to be the precursor proteins or the large segments of active peptides, which both proteins may play the important role in maintaining the physiological function of BG. Both LSBG and RSBG in NLCS can express the differential proteins induced with the cadmium under the stress of actual cadmium chloride at 10 μg/mL. However, these proteins were effectively separated, selected and identified with proteomic techniques, indicating that these proteins were considered to be the down-regulated proteins such as ropomyosin and M_w=16000 calcium-binding, and the up-regulated proteins such as heat shock protein and thioredoxin. We suggest that these proteins be tightly connected with mithridatism of cadmium in BG cells and , as protein markers in part, be suit for monitoring pollution level of cadmium and developing the research focused on toxicology.

Key words: Aplysia, Sub-baccual ganglion, Proteomics, Cadmium chloride, Protein marker