Saccharified Thermoresponsive Modification of Polystyrene and Its Effect on Cell Behavior

Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (12): 2502.

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Saccharified Thermoresponsive Modification of Polystyrene and Its Effect on Cell Behavior

HE Xiao-Ling^1,2, DONG You-Yu¹, NIE Ping-Ping¹, FENG Xia¹, CHEN Li^1*

1.Tianjin Key Laboratory of Fiber Modification and Functional Fiber, School of Materials Science and Engineering,
2. School of Environment and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300160, China

Received:2009-03-19 Online:2009-12-10 Published:2009-12-10
Contact: CHEN Li. E-mail: tjpuchenlis@163.com
Supported by:
国家自然科学基金(批准号: 50973084)、天津市应用基础及前沿技术研究计划项目(批准号: 09JCZDJC23100)和天津市高等学校科技发展基金(批准号: 20071214)资助.

Abstract

Abstract:

Poly(N-isopropylacrylamide) and acrylic acid were grafted onto tissue culture polystyrene(TCPS) by UV irradiation, followed by immobilization of the monoamine terminated lactobionic(L-NH₂) to acrylic acid carboxyls. The compositions of modified surfaces were characterized by ATR-FTIR and XPS. The morphologies were observed via AFM. Results from drop contact angle measurement indicate that the modified surfaces show hydrophobic/hydrophilic property alterations in response to temperature. Adhesion and detachment of hepatic tumor cells(HepG-2) were examined on virgin surface and modified surfaces. Immobilization of L-NH₂ facilitated HepG-2 cells adhesion on saccharified thermoresponsive surface. Moreover, HepG-2 cells cultured on modified surfaces can be recovered non-invasively by decreasing temperature.

Key words: Poly(N-isopropylacrylamide); Galactose; Temperature sensitivity; Cell culture; Cell detachment

TrendMD:

HE Xiao-Ling, DONG You-Yu, NIE Ping-Ping, FENG Xia, CHEN Li*. Saccharified Thermoresponsive Modification of Polystyrene and Its Effect on Cell Behavior[J]. Chem. J. Chinese Universities, 2009, 30(12): 2502.

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Saccharified Thermoresponsive Modification of Polystyrene and Its Effect on Cell Behavior

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