Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (12): 2376.

• Articles • Previous Articles     Next Articles

Study on the Target Interaction Between Mitochondria and Signal Peptide Functionalized Silica Nanoparticles

HE Xiao-Xiao, YUAN Yuan, SHI Hui, WANG Ke-Min*, HE Ding-Gen, QIN Di-Lan   

  1. Biomedical Engineering Center, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Institute of Life Science and Biotechnology, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Hunan University, Changsha 410082, China
  • Received:2009-07-15 Online:2009-12-10 Published:2009-12-10
  • Contact: WANG Ke-Min. E-mail: kmwang@hnu.cn
  • Supported by:

    国家“九七三”计划项目(批准号: 2002CB513100-10)、教育部科学技术重点项目(批准号: 107084)、新世纪优秀人才支持计划(批准号: NCET-06-0697)和国家自然科学基金(批准号: 90606003, 20775021)资助.

Abstract:

The mitochondrial signal peptide functionalized fluorescent silica nanoparticles were prepared by conjugated the mitochondrial signal peptide to the nanoparticles through N-(p-maleimidophenyl) isocyanate(PMPI). The fluorescence spectrophotometer, zeta potential and TEM were used to characterize the fluorescent silica nanoparticles. The results show that the signal peptides are successfully conjugated to the surface of fluorescent silica nanoparticles, and the size of the fluorescent silica nanoparticles do not change obviously before and after modification. With the isolated nucleus as a control, the target interaction between the mitochondrial signal peptides modified fluorescent silica nanoparticles and the isolated mitochondria was investigated by flow cytometry. It was revealed that the bioactivity of the signal peptide was well remained after the conjugation with the silica nanoparticles, and the peptides could facilitate the specific recognition and binding between the silica nanoparticles and the isolated mitochondria, which put forwards a new idea for the monitoring and function controlling of the mitochondria.

Key words: Silica nanoparticle; Mitochondrial signal peptide; Mitochondria

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