CJCU

Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (12): 2331.

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Synthesis and Characterization of APTTS-Fe3O4 Nanoparticles for in vitro Transfection as Gene Carriers

LI Wen-Zhang1, LI Jie1*, TAN Ze-Ming2   

  1. 1. College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China;
    2. Neurosurgery Department of Xiang-Ya Hospital, Central South University, Changsha 410078, China
  • Received:2009-03-06 Online:2009-12-10 Published:2009-12-10
  • Contact: LI Jie. E-mail： lijieliu@mail.csu.edu.cn
  • Supported by:

    教育部新世纪优秀人才资助计划(批准号： NCET-05-0691)和湖南省重大科技计划项目(批准号： 2008SK1001)资助.

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Abstract:

Fe3O4 magnetic nanoparticles were prepared via thermal decomposition of Fe(acac)3 in 2-pyrrolidone. And 3-aminopropyltriethoxy-silane(APTTS) was used to modify the surface of the nanoparticles. The structure and properties of the samples were characterized by means of XRD, TEM and FTIR. The potency of adsorbing DNA and resisting DNase-I digestion of APTTS/Fe3O4 were analyzed via agarose gel electrophoresis. Then, APTTS/Fe3O4 nanoparticles were evaluated as a kind of plasmid pCMVCD carrier and transferred into human glioma cell line U251 in vitro. The mRNA and protein expression of intracellular CD gene were tested by RT-PCR, Western blot and immunofluorescent staining, respectively. The results show that all the Fe3O4 nanoparticles are sized 8—10 nm and have a good crystallinity. Displaying functional group of —OH, —NH, —NH2, —C—O, —C—OH, the amino-silane modified nanoparticles had the ability of binding DNA and resisting DNase-I digestion. Though the cell transfect test, such composite nanoparticles can transfer DNA into U251 cells efficiently and get stable expression.

Key words: Fe3O4 nanoparticle; Magnetic material; In vitro transfection; Gene carrier

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