Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (11): 2170.

• Articles • Previous Articles     Next Articles

Ultrasensitive Electrochemical Assay for the Detection of p53 Sequence Based on the Aggregation of Gold Nanoparticles

ZHENG Jing1,2, SHEN Bi-Jun1, CHENG Gui-Fang1*, FENG Wan-Juan1, HE Pin-Gang1, FANG Yu-Zhi1   

  1. 1. Department of Chemistry, East China Normal University, Shanghai 200062, China;
    2. Department of Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China
  • Received:2009-03-12 Online:2009-11-10 Published:2009-11-10
  • Contact: CHENG Gui-Fang. E-mail: gfcheng@chem.ecnu.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 20675031)、上海市科委浦江人才计划(批准号: 06PJ14032)和上海工程技术大学校发展基金(批准号: 2008xy32)资助.

Abstract:

An ultrasensitive and high specific electrochemical assay for the detection of human tumor suppression p53 gene has been developed. Two DNA sequences were designed, and the combined sequences were complementary to that of the target p53 sequence, one was immobilized on the magnetic nanoparticles for capturing the target p53 sequence, the other was labeled with gold nanoparticles as probe. The ultrasensitive detection was achieved by the aggregation of gold nanoparticles with introducing thiocyanuric acid into the system to form the network like structure, and the detection limit could go down to 2.24×10-17 mol/L for the wild type p53 sequence. The discrimination between the mutant type p53 DNA and the wide type p53 DNA was studied with the proposed assay.

Key words: Gold nanoparticle; p53 Gene; Electrochemical detection

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