Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (10): 2009.

• Letters • Previous Articles     Next Articles

Synthesis and ESR Study of Covalent Connection of EMPO on L-Tyr

DU Li-Bo, WANG Guang-Qing, HAN Lu, JIA Hong-Ying, TIAN Qiu, LIU Yang*   

  1. State Key Lab for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
  • Received:2009-03-24 Online:2009-10-10 Published:2009-10-10
  • Contact: LIU Yang. E-mail: yliu@iccas.ac.cn
  • Supported by:

    国家自然科学基金(批准号: 90813021, 20875093)资助.

Abstract:

L-Tyr-EMPO, a new EMPO analogue bearing an L-tyrosine methyl ether group, was first synthesized by acylation. Various radicals, including O2, ·OH, ·OR, and ·R, have been efficiently detected and characterized via L-Tyr-EMPO. The half-life of the L-Tyr-EMPO superoxide adduct was estimated to be ca. 6.5 min. More importantly, the present study demonstrated a new synthetic strategy for covalent conjugation between cyclic-nitrone and amino group in peptides or proteins, by which the site-specifically spin trapping can be performed via antibody linked nitrone in the near future. Furthermore, with the help of the covalent link, the targeting for the areas of interest in which the monitored radical species was sitespecially generated.

Key words: Spin trapping; Bioconjugate; Superoxide anion; Nitrone; Free radical

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