Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (10): 2003.

• Articles • Previous Articles     Next Articles

New Method of Soybean Seed Ferritin Purification and Comparision of Activity with Pea Seed Ferritin

HU Ju, LIAO Xia-Yun, DENG Jian-Jun, HU Xiao-Song, ZHAO Guang-Hua*   

  1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • Received:2009-02-16 Online:2009-10-10 Published:2009-10-10
  • Contact: ZHAO Guang-Hua. E-mail: gzhao1000@yahoo.com
  • Supported by:

    国家“八六三”计划(批准号: 2007AA10Z333)资助.

Abstract:

Ferritin is a class of iron storage protein presents in all living organisms and serves the dual function of iron detoxification and iron storage. In this paper, a new method of soybean seed ferritin purification was reported. Crude extracts of soybean were heated at 55 ℃ for 15 min, then adjusted to 500 mmol/L MgCl2, stored for 30 min at 4 ℃. It was followed by addition of sodium citrate to final concentration of 700 mmol/L. The protein was further purified by ion exchange chromatography in DEAE-Sepharose and Sephacryl S-300 gel filtration columns. Native-PAGE purified the molecular weight of soybean ferritin was about 560000 and SDS-PAGE analyzed ferritin subunits were separated as two peptides(28000 and 26500). Subsequently, we compared the iron oxidative deposition and reductive release activities of soybean ferritin with those of pea seed ferritin, and found that their mechanisms of the iron oxidative deposition and iron release are different. Finally, a possible reason was given.

Key words: Soybean seed; Ferritin; Purification; Activity identification

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