CJCU

Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (10): 1998.

• Articles • Previous Articles     Next Articles

Studies on the Purification, Infrared Spectroscopic Analysis and Antiangiogenesis Effects of Fibrinolyric Protein(EFP) from Eupolyphage sinesis Walker

LI Sui-Jing, HAN Ya-Li*, ZHANG Dong-Mei, LIU Hao, LI Zi-Wei   

  1. Bioengineering Department, Faculty of Light and Chemical, Guangdong University of Technology, Guangzhou 510006, China
  • Received:2009-01-13 Online:2009-10-10 Published:2009-10-10
  • Contact: HAN Ya-Li. E-mail: ylhan57@126.com
  • Supported by:

    国家自然科学基金(批准号: 30772739)资助.

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Abstract:

A novel of fibrinolytic protein(EFP) was separated and purified by homogenate, salting-out, ammonium sulfate fractionation, DEAE-52 cellulose column chromatography from fresh tradional chinese medicine Eupolyphaga sinensis Walker. The molecular weight and purity of EFP were detected by sulfate-polyacry-lamide gel electrophoresis analysis. Thin polyacrylamide fibrin plate assay was used to test the fibrinolysin activity of EFP. The key functional group of EFP was analyzed by infrared spectroscopy. Chicken embryo chorioallantoic membrane(CAM) assay was used to test the inhibited effects of EFP on neovascularization in vivo. The results show that the molecular weight of fibrinolyric proteins from Eupolyphaga sinensis is approximately 41300 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, the infrared spectra of the tablets showed characteristic of protein and glycoprotein. The vascular network of CAM of chicken embryo is inhibited by EFP. The results indicate that EFP possesses anti-angiogenic activity and may be useful for the development of novel anti-cancer therapy.

Key words: Fibrinolyric protein of Eupolyphaga sinensis Walker(EFP); Angiogenesis; Infrared spectroscopy

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