Chem. J. Chinese Universities

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Analysis of Geniposide and Total Nitrogen Content in Qingkailing Injection Intermediate by Near Infrared Spectroscopy and Synergy Interval Partial Least Squares

ZHU Xiang-Rong1,2, LI Na2, SHI Xin-Yuan2, QIAO Yan-Jiang2, ZHANG Zhuo-Yong1*   

    1. Department of Chemistry, Capital Normal University, Beijing100037, China;
    2. School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China
  • Received:2007-10-25 Revised:1900-01-01 Online:2008-05-10 Published:2008-05-10
  • Contact: ZHANG Zhuo-Yong

Abstract: Near infrared spectrometry combined with chemometric methods was used for establishing a new method to determine the content of total nitrogen and geniposide in Qingkailing injections. The training and testing sets are partitioned by Kernard-Stone algorithm. Synergy interval partial least squares(siPLS) is used for selecting effective spectral regions and building the quantitative calibration models of the total nitrogen and geniposide. Pre-treatments of spectra are discussed in detail. Correlation coefficient obtained is 0.999, RMSECV is 0.023, RMSEP is 0.074 for the total nitrogen, and correlation coefficient is 0.708, RMSECV is 0.023, RMSEP is 0.159 for geniposide, respectively. The predictive results show that the proposed method is rapid, non-destructive and credible, which can be applied to control the quality of Chinese medicine injection online in industry scenes extensively.

Key words: Near infrared spectroscopy, Qingkailing injection intermediate, Kernard-Stone algorithm method, Synergy interval partial least squares(siPLS), Online control

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