CJCU

Chem. J. Chinese Universities ›› 2006, Vol. 27 ›› Issue (2): 222.

• Contents • Previous Articles     Next Articles

Comparison Studies of Constituents Between Dangguibuxuetang and
Its Single Medicines via Hyphenated Chromatography and
Multicomponent Spectral Correlative Chromatography

WANG Ya-Li 1,2,  LIANG Yi-Zeng 1,  HU Yun 3,  LI Bo-Yan1, ZENG Zhong-Da 1,  XU Shu-Ping4   

  1. 1. Research Center of Modernization of Chinese Herbal Medicines, Central South University, Changsha 410083, China;
    2. Department of Pharamacy, Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China;
    3. Department of Chemistry, School of Science & Technology, Kwansei-Gakuin University, Sanda 669-1337, Japan;
    4. Key Laboratory for Supermolecular Structure and Materials of Ministry of Education,
    Jilin University,  Changchun 130023, China
  • Received:2005-04-30 Online:2006-02-10 Published:2006-02-10
  • Contact: LIANG Yi-Ceng,E-mail: yizeng_liang@263.net

PDF (PC)

Abstract:

AbstractIn this study, a compound medicine of Dangguibuxuetang and its two involved single medicines of Danggui(Radix Angelica sinensis) and Huangqi(Radix Astragali) were compared for their relevant constituents after the combination of hyphenated chromatography and multicomponent spectral correlative chromatography, based on the two-dimensional spectrochromatographic fingerprints of the three medicinal objects obtained from HPLC-DAD. It was found that of twenty-one high peaks in Dangguibuxuetang, eight originate from Radix Angelica sinensis, six from Radix Astragali, and seven from both Radix Angelica sinensis and Radix Astragali. A little of other components in the investigated overlapping peaks varied. Moreover, new chemical ingredients appeared just in spectrochromatographic fingerprints of Dangguibuxuetang, and some existing in those of single medicines contrarily disappeared after the compatibility, as opposite in Radix Angelica sinensis and Radix Astragali. It suggested that there are certain interactions of the chemical ingredients in compound medicine besides their total sum effect of single medicines. This proposed approach was quite convenient and reliable to make a quick comparison and analysis of the relevant constituents in spectrochromatographyic fingerprints of the compound and single medicines, especially for the comparison of two overlapping peaks.

Key words: KeywordsHyphenated chromatography; Multicomponent spectral correlative chromatography; Dangguibuxuetang; Single medicine; Component comparison

CLC Number: 

TrendMD: