CJCU

Chem. J. Chinese Universities ›› 2002, Vol. 23 ›› Issue (5): 809.

• Articles • Previous Articles     Next Articles

Accelerator Mass Spectrometry Studies on Adduction of Nicotine with Hemoglobin and Serum Albumin in Mouse

WANG Yan1, WANG Hai-Fang1, SUN Hong-Fang1, WANG Xiang-Yun1, LIU Yuan-Fang1, LIU Ke-Xin2, LU Xiang-Yang2, MA Hong-Ji2, LI Kun2   

  1. 1. College of Chemistry and Molecular Engineering;
    2. Institute of Heavy Ion Physics, School of Physics, Peking University, Beijing 100871, China
  • Received:2001-03-06 Online:2002-05-24 Published:2002-05-24

PDF (PC)

Abstract: Adducts of hemoglobin(Hb) and serum albumin(SA) with nicotine in mouse blood were mea-sured respectively by using accelerator mass spectrometry (AMS).The dose response of adduct levels ofHb/SAand the correlative relationship between the two blood protein adducts were investigated over arange of doses(0.1-100.0 μg/kg body weight), which were equivalent to a low-level dose of environ-mental exposure of cigarette smoking.Both the levels of Hb and SAadducts increased with increasing doseand showed a linear relationship.By plotting Hb/SAadducts versus DNAadducts, a linear relationship fitsthe experimental observations best, which means that Hb/SAadduct could substitute for DNAadduct as abiomarker for nicotine.The detection limit of protein adduct in this work is 0.03 pmol/g protein(namely3.2 adducts/1012 amino acid residues), the excellent sensitivity in protein biomarker measurements com-pared to the data reported previously.

Key words: Accelerator mass spectrometry(AMS), Nicotine, Hemoglobin (Hb) adduct, Serum albumin (SA) adduct

CLC Number: 

TrendMD: