CJCU

Chem. J. Chinese Universities ›› 2001, Vol. 22 ›› Issue (6): 901.

• Articles • Previous Articles     Next Articles

Determination of Nucleic Acids at Nanogram Levels with Tetra-substituted Amino Aluminum Phthalocyanine by a Resonance Light-scattering Technique

CHEN Xiao-Lan1, LI Dong-Hui2, ZHU Qing-Zhi1, YANG Huang-Hao1, ZHENG Hong1, XU Jin-Gou1   

  1. 1. Department of Chemistry, the Key Laboratory of Analytical Sciences of MOE;
    2. Cancer Research Center, Xiamen University, Xiamen 361005, China
  • Received:2000-07-13 Online:2001-06-24 Published:2001-06-24

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Abstract: This is the first report of the determination of nucleic acids at nanogram levels based on their enhanced effects on the resonance light-scattering of TAAlPc at 400 nm. Under optimal conditions, the linear ranges of the calibration curves were 0—2500 ng/mLfor Calf thymus DNA(CT DNA), 0—200.0 ng/mLfor Salmon sperm DNA(SM DNA) and 0—4000 ng/mLfor Yeast RNA. The detection limits were 1.4 ng/mLfor both CT DNAand SM DNA, 2.7 ng/mLfor Yeast RNA. The relative standard deviation for the determination of 100.0 ng/mL CT DNAwas 2.1%. The measurement can be made easily on a common spectrofluorimeter. The reaction between TAAlPc and nucleic acids is completed in 2 min and the scattered light signal is stable for at least 1 h. The interference from some interesting co existing substances with the determination of DNAwas also examined. The method was applied to the determination of DNAin golden staphylococcus DNAsample, and the results were satisfactory.

Key words: Tetra substituted amino aluminum phthalocyanine, Nucleic acids, Resonance light scattering

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