Abstract:

2-Keto-L-gulonic acid (for short gulonic acid), which was produced with pioneering twice fermentation technique in China, is an intermediate of the production of Vitamin C. The impurities (protein, mycelium et al.) in the fermentation liquor can be removed by heat precipitation of the original process, however, gulonic acid is heat-sensitive, its yield will be reduced when heating. The ultrafiltration membrane separation technique has been used to extract gulonic acid from Vitamin C fermentation liquor^[1], which is operated at room temperature, with a pretreatment of the fermentation liquor before ultrafiltration, to avoid the jam of membrane.