Studies on Separation of Derivatives of Amino Acid of Enantiomers on Chiral Stationary Phase by High Performance Liquid Chromatography

Chem. J. Chinese Universities ›› 1995, Vol. 16 ›› Issue (7): 1072.

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Studies on Separation of Derivatives of Amino Acid of Enantiomers on Chiral Stationary Phase by High Performance Liquid Chromatography

SHEN Han-Xi¹, YANG Guo-Sheng¹, GAO Ru-Yu², WANG Qin-Sun²

1. Department of Chemistry, Nankai University, Tianjin, 300071;
2. Institute of Elemento-Organic Chemistry, Nankai University, Tianjin, 300071

Received:1994-06-04 Revised:1995-02-27 Online:1995-07-24 Published:1995-07-24

Abstract

Abstract: Eighteen derivatives of six DLamino acid alanine, 2-amino-n-butanoic acid, va-line, leucine, phenylalanine, methionine of enantiomers were separated by L-isoleucine-t-butylamide type chiral bonded stationary phase (CSP) in HPLC. The experimental result showed that the chiral stationary phase had good resolution for the racemic α-amino acid derivatives. The performance of the structure of derivatives and composition of mobile phase for the resolution was examined. The probable mechanism for chiral recognition was proposed.

Key words: Amino acids, Chiral bonded stationary phase, Enantiomer separation

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SHEN Han-Xi, YANG Guo-Sheng, GAO Ru-Yu, WANG Qin-Sun. Studies on Separation of Derivatives of Amino Acid of Enantiomers on Chiral Stationary Phase by High Performance Liquid Chromatography[J]. Chem. J. Chinese Universities, 1995, 16(7): 1072.

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Studies on Separation of Derivatives of Amino Acid of Enantiomers on Chiral Stationary Phase by High Performance Liquid Chromatography

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