Abstract: Endogenous opioid deltorphinⅠ(DELⅠ),Ⅱ(DELⅠ)and their five analogues ([L-Ala²]DEL Ⅰ,[L-Ala²]DEL Ⅱ,[Val⁴,Asp⁵]DELⅠ,[Val⁴,Asp⁶]DELⅠ,[Gly⁴,Asp⁷]DELⅠ)were designed on a rational basis and were synthesized by solid phase peptide synthesis method.The synthesis was performed manually on BHAresin prepared by ourselves,DCC was used as coupling reagent and TFAas deboc reagent. Peptide-resin was cleavaged by HF, the crude peptides were obtained after lyophylization. The crude peptides were desalted on a sephadex Glo column, then the pure peptides were obtained by partitional chromatography on a sephadex G25 column. The purities of all synthetic peptides were greater than 95%, which were acquired by reversed HPLC analysis. The data we got from FABMS,AAA and sequence analysis were in agreement with the caculated values.

Key words: Deltorphins, Solid phase peptide synthesis, Opioid, Design of analogues