Abstract: The interaction of DDPwith proteins of erythrocyte membrane was studied. When DDPwas incubated with membranes for 24 h at 37℃, the bound DDPcontent per mg membrane proteins was 0. 11 μmol. The distribution and polymeric form of membrane proteins were investigated by SDS-PAGE method, DDP made band 1,2(spectrin) polymerize. The distribution of DDPon membrane proteins was determined by flameless atomic absorption spectroscopy, all the membrane proteins bind with DDP. It was observed that DDP decreased the sulfhydryl content of membranes. Sulfhydryl groups are one of binding sites for DDP. DDP can cause change of S/W in ESRspectrum of maleimide labeled erythrocyte membranes. This indicates that DDP affects the conformation of membrane proteins. These phenomena support our postulation that membrane proteins are the target of DDPin cell membranes.

Key words: cis-Dichlorodiammineplatinum, Erythrocyte, Membrane-protein