Chem. J. Chinese Universities ›› 2021, Vol. 42 ›› Issue (11): 3321.doi: 10.7503/cjcu20210420

• Review • Previous Articles     Next Articles

Recent Advance in Light-controlled CRISPR Technology

LIU Hong1, JIANG Jinghong2, DUAN Zhijuan1, XU Shijun1, HUANG Fujian1(), XIA Fan1   

  1. 1.Faculty of Materials Science and Chemistry,China University of Geosciences,Wuhan 430074,China
    2.Obstetrics & Gynecology Department of Zhongnan Hospital,Wuhan University,Wuhan 430071,China
  • Received:2021-06-21 Online:2021-11-10 Published:2021-08-18
  • Contact: HUANG Fujian E-mail:huangfj@cug.edu.cn
  • Supported by:
    the National Natural Science Foundation of China(21974127)

Abstract:

The CRISPR-Cas system provides a versatile tool for programmable genome editing. The collision of CRISPR with optogenetics and photochemical biology has produced a brilliant spark. The photoactivated CRISPR-Cas system enables better spatial and temporal regulation of RNA-guided nuclease activity. In recent years, scientists have developed a range of light-activated CRISPR tools using a combination of CRISPR and a variety of optical techniques. These tools allow researchers to conduct high-resolution studies of life activities in spatial, temporal and genomic coordinates. In this review, research progress of the CRISPR system, gene editing technology, optogenetics and photochemical biology are briefly summarized, and the development of light-induced CRISPR technology is prospected.

Key words: Photoswitching, Photocaging, CRISPR-Cas system, Genome editing

CLC Number: 

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