Abstract: An innovative method of preparing immobilized Laccase-Mediator System (Im-LMS) was established by immobilizing Trametes versicolor laccase and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) on cellulose microspheres simultaneously. The mediator ABTS was mixxed with ionic liquid cellulose solution and was encapsulated during the process of cellulose regeneration. The as-prepared cellulose-ABTS microspheres(Ce/ABTS) with 3D pore structure were characterized by SEM, XRD and FT-IR. Then laccase was immobilized on Ce/ABTS by dopamine self-polymerization to synthesize Ce/ABTS@Do/Lac. The method developed in this work, for the first time, realized the co-immobilization of laccase and mediator facilely and effectively. The Ce/ABTS@Do/Lac were used in the enzymatic degradation of indole, and the degradation rate was up to 99%. As compared with the free laccase, the Ce/ABTS@Do/Lac showed higher pH stability and operational stability.Benefited from the mediation effect of the immobilized ABTS, the Ce/ABTS@Do/Lac still worked after 9 cycling runs, which was 9-fold longer than that of a free laccase-mediator system. The successful recovery of both laccase and mediator is promising to reduce the cost for the application of laccase in wastewater treatment and might be helpful to cut down the secondary pollution from free laccase mediators. The Ce/ABTS@Do/Lac must have a great potential in large-scale application for organic wastewater treatment.