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Chem. J. Chinese Universities ›› 2012, Vol. 33 ›› Issue (07): 1394.doi: 10.3969/j.issn.0251-0790.2012.07.006

• Preface • Previous Articles     Next Articles

Construction of Bio-functional Nanosized Silica Microspheres for Capture and Separation of Glutathione S-Transferase

ZOU Xue-Yan1,3, CHU Liu-Jie2, DONG Shuo2, ZHAO Yan-Bao1, LI Bin-Jie2   

  1. 1. Key Laboratory for Special Functional Materials, Henan University, Kaifeng 475004, China;
    2. Key Laboratory of Cellular and Molecular Immunology, Henan University, Kaifeng 475004, China;
    3. Basic Experiment Teaching Center, Henan University, Kaifeng 475004, China
  • Received:2011-09-19 Online:2012-07-10 Published:2012-07-10

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Abstract: Nanosized silica microspheres with sulfydryl group(nSiO2-SH) were synthesized with(3-mercaptopropyl)trimethoxysilane(MPS) via one-step hydrolyzing process, and the effects of volume ratio of water to ethanol, reaction temperature, reaction time and the volume of MPS on the product morphologies were discussed. Additionally, the possible reaction mechanism was also proposed. The prepared nSiO2-SH microspheres reacted further with glutathione through disulfide bond(-S-S-) to produce nSiO2-GSH microspheres. Their morphology, size and ingredient were characterized by scanning electron microscopy(SEM), transmission electron microscopy(TEM), Fourier transform infrared spectrometry(FTIR) and thermogravimetric(TG) analysis. The behavior of capture and separation of glutathione S-transferase(GST) was detected by sodium dodecylsulfate-polyacrylamide gel electrophoresis(SDS-PAGE) method. The results show that GST can be specifically adsorbed on nSiO2-GSH microspheres in the mixed protein solution. Therefore, nSiO2-GSH microspheres have potential applications in the separation, purification and detection of the GST-tagged fusion proteins.

Key words: Silica with sulfydryl group, Bio-functionalization, Specific adsorption, Separation of glutathione S-transferase

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