Construction of Biomimetic Cross-linking Polyplexes with Thiolated-HA Shielding

doi:10.3969/j.issn.0251-0790.2012.02.034

Abstract

Abstract: The preparation of biomimetic cross-linking polyplexes was an effective way to solve the contradiction between extracellular stability and intracellular dissociation of polyplexes. In this research, thiolated-HA(hyaluronic acid) was synthesized and then added to PEI[poly(ethleneimine)]/DNA polyplexes. Due to the electrostatic attraction, cross-linking polyplexes via disulfide bonds were constructed. Gel retardation assay indicated that these ternary polyplexes showed efficient DNA condensation ability. The biomimetic cross-linking shell improved the stability in physiological salt condition. The in vitro cell culture indicated that the cross-linking polyplexes reduced cytotoxicity and showed efficient transfection efficiency. This facile approach to constructing biomimetic cross-linking polyplexes might have great potential application in non-viral gene delivery research.

Key words: Non-viral gene vector, Biomimetic cross-linking polyplex, Supramolecular assembly, Poly(ethy-leneimine)(PEI), Hyaluronic acid(HA)

CLC Number:

O631

TrendMD:

XU Zhi-Xue, YANG Lu, TANG San, HU Qiao-Ling, WANG You-Xiang. Construction of Biomimetic Cross-linking Polyplexes with Thiolated-HA Shielding[J]. Chem. J. Chinese Universities, 2012, 33(02): 404.

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