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Time-Resolved Spectral Analysis of Ultrafast Isomerization Dynamics of Retinal in Bacteriorhodopsin

WU Yi-Shi1,2, ZHONG Sheng3, AI Xi-Cheng2, HU Kun-Sheng3, ZHANG Jian-Ping2*   

    1. Beijing National Laboratory for Molecular Science(BNLMS), State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China;
    2. Department of Chemistry, College of Science, Renmin University of China, Beijing 100872, China;
    3. Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
  • Received:2007-05-30 Revised:1900-01-01 Online:2008-01-10 Published:2008-01-10
  • Contact: ZHANG Jian-Ping

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Abstract: The ultrafast photoisomerization process of retinal in Bacteriorhodpsin's(BR) photocycle was investigated via femtosecond time-resolved absorption spectroscopy. Singular value decomposition(SVD) combined with model-based global fitting was employed to analyze the time-resolved spectra, and the related difference spectra(SADS) and the population dynamics of I460, J625 and K590 intermediates were derived on the basis of our proposed reaction scheme. The detailed photoisomerization pathways were discussed. Moreover, the SADS of Franck-Condon state(H intermediate), radiative transition of which accounts for the stimulated emission around 700 nm, was also detected, and its time constant was 0.04 ps. Our results prove the involvement of the H-intermediate in ultrafast photoisomerization process of retinal in BR.

Key words: Bacteriorhodopsin, Ultrafast isomerization, Singular value decomposition, Stimulated emission

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